Research Laboratory on Urine, Unità Operativa di Nefrologia, Dialisi, Trapianto, Fondazione IRCCS Ca' Granda-Ospedale Maggiore Policlinico, Milano, Italy.
Clin Chim Acta. 2010 Jun 3;411(11-12):859-67. doi: 10.1016/j.cca.2010.02.073. Epub 2010 Mar 7.
EQA programs on urinary sediment are rare. We describe an EQA Italian program which started in 2001 and involves today more than 300 laboratories.
The program, which started with a questionnaire about the methodological aspects on urinary sediment, includes today four surveys per year. These ask the participants the identification and clinical associations of urinary sediment particles shown by colour images (surveys 1 and 3) and the diagnosis of clinical cases presented by both images and a short clinical history (surveys 2 and 4). The results of each survey are then scored and commented.
Questionnaire (participants = 287): most methodological aspects were not dealt with properly.
cells, lipids, casts and some contaminants were poorly known. However, when 27 particles were presented for the second time and 16 particles for the third time, the correct identification rate for most of them increased significantly. Clinical associations (No presented = 16): a correct answer was indicated by > or = 84% of participants for all particles but one. Clinical cases (No presented=4): lowest correct identification for urine contamination from genital secretion (77.3%), highest for ureteric stone (94.4%).
Our program shows that EQA programs are both useful and needed.
尿液沉淀物的室间质量评价(EQA)方案很少。我们描述了一个始于 2001 年的意大利 EQA 方案,目前已有超过 300 家实验室参与。
该方案最初采用一份有关尿液沉淀物方法学方面的问卷,目前每年包括四次调查。这些调查要求参与者识别并将尿液沉淀物彩色图像上显示的颗粒与临床联系起来(调查 1 和 3),并根据图像和简短的临床病史诊断临床病例(调查 2 和 4)。然后对每个调查的结果进行评分和评论。
问卷(参与者=287):大多数方法学方面没有得到妥善处理。
细胞、脂质、管型和一些污染物的识别率较低。然而,当 27 个颗粒被第二次呈现,16 个颗粒被第三次呈现时,大多数颗粒的正确识别率显著提高。临床关联(未呈现=16):除了一个颗粒外,所有颗粒的正确答案均由≥84%的参与者指出。临床病例(未呈现=4):尿液受生殖器分泌物污染的识别率最低(77.3%),输尿管结石的识别率最高(94.4%)。
我们的方案表明,EQA 方案既有用又有必要。