哺乳动物 NEIL1 蛋白在 DNA 中 8-氧-7,8-二氢腺嘌呤修复中的作用。
The role of mammalian NEIL1 protein in the repair of 8-oxo-7,8-dihydroadenine in DNA.
机构信息
SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
出版信息
FEBS Lett. 2010 Apr 16;584(8):1553-7. doi: 10.1016/j.febslet.2010.03.009. Epub 2010 Mar 7.
Abstract
8-oxo-7,8-dihydroadenine (8-oxoAde) is a major product of adenine modification by reactive oxygen species. So far, only one mammalian DNA glycosylase, 8-oxoguanine-DNA-glycosylase 1 (OGG1), has been shown to excise 8-oxoAde, exclusively from pairs with Cyt. We have found that endonuclease VIII-like protein 1 (NEIL1), a mammalian homolog of bacterial endonuclease VIII, can efficiently remove 8-oxoAde from 8-oxoAde:Cyt pairs but not from other contexts. In an in vitro reconstituted system, reactions containing OGG1 produced a fully repaired product, whereas NEIL1 caused an abortive initiation of repair, stopping after 8-oxoAde removal and DNA strand cleavage. This block was partially relieved by polynucleotide kinase/3'-phosphatase. Thus, two alternative routes of 8-oxoAde repair may exist in mammals.
摘要
8-氧代-7,8-二氢腺嘌呤（8-氧代腺嘌呤）是活性氧物种修饰腺嘌呤的主要产物。到目前为止，只有一种哺乳动物 DNA 糖苷酶，8-氧代鸟嘌呤-DNA-糖苷酶 1（OGG1），被证明可以从 Cyt 配对中专门切除 8-氧代腺嘌呤。我们发现，内切核酸酶 VIII 样蛋白 1（NEIL1），一种细菌内切核酸酶 VIII 的哺乳动物同源物，可以有效地从 8-氧代腺嘌呤：Cyt 对中去除 8-氧代腺嘌呤，但不能从其他情况下去除。在体外重建的系统中，含有 OGG1 的反应产生了完全修复的产物，而 NEIL1 导致修复的无效起始，在 8-氧代腺嘌呤去除和 DNA 链断裂后停止。多核苷酸激酶/3'-磷酸酶部分缓解了这种阻断。因此，8-氧代腺嘌呤的修复可能存在两种替代途径。
相似文献
1
The role of mammalian NEIL1 protein in the repair of 8-oxo-7,8-dihydroadenine in DNA.哺乳动物 NEIL1 蛋白在 DNA 中 8-氧-7,8-二氢腺嘌呤修复中的作用。
FEBS Lett. 2010 Apr 16;584(8):1553-7. doi: 10.1016/j.febslet.2010.03.009. Epub 2010 Mar 7.
2
Futile short-patch DNA base excision repair of adenine:8-oxoguanine mispair.腺嘌呤：8-氧代鸟嘌呤错配的无效短片段DNA碱基切除修复
Nucleic Acids Res. 2004 Nov 5;32(19):5928-34. doi: 10.1093/nar/gkh909. Print 2004.
3
Effect of DNA Glycosylases OGG1 and Neil1 on Oxidized G-Rich Motif in the Promoter.DNA 糖苷酶 OGG1 和 Neil1 对启动子中氧化的 G-富基序的影响。
Int J Mol Sci. 2021 Jan 24;22(3):1137. doi: 10.3390/ijms22031137.
4
Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine.碱基切除修复蛋白对 8-氧代-7,8-二氢鸟嘌呤（8-羟基鸟嘌呤）与胞嘧啶和腺嘌呤配对引发突变的影响。
DNA Repair (Amst). 2010 May 4;9(5):542-50. doi: 10.1016/j.dnarep.2010.02.004. Epub 2010 Mar 1.
5
NEIL1 and NEIL2 Are Recruited as Potential Backup for OGG1 upon OGG1 Depletion or Inhibition by TH5487.当OGG1被TH5487耗尽或抑制时，NEIL1和NEIL2作为OGG1的潜在备用蛋白被招募。
Int J Mol Sci. 2021 Apr 27;22(9):4542. doi: 10.3390/ijms22094542.
6
Coordination of DNA repair by NEIL1 and PARP-1: a possible link to aging.NEIL1与PARP-1对DNA修复的协同作用：与衰老的可能联系。
Aging (Albany NY). 2012 Oct;4(10):674-85. doi: 10.18632/aging.100492.
7
Requirements for DNA bubble structure for efficient cleavage by helix-two-turn-helix DNA glycosylases.DNA 双螺旋-两转-双螺旋 DNA 糖苷酶有效切割所需的 DNA 泡结构。
Mutagenesis. 2020 Feb 13;35(1):119-128. doi: 10.1093/mutage/gez047.
8
AP endonuclease-independent DNA base excision repair in human cells.人类细胞中不依赖脱嘌呤嘧啶内切酶的DNA碱基切除修复
Mol Cell. 2004 Jul 23;15(2):209-20. doi: 10.1016/j.molcel.2004.06.003.
9
Stimulation of DNA glycosylase activity of OGG1 by NEIL1: functional collaboration between two human DNA glycosylases.NEIL1对OGG1 DNA糖基化酶活性的刺激：两种人类DNA糖基化酶之间的功能协作。
Biochemistry. 2004 Sep 14;43(36):11596-604. doi: 10.1021/bi049097i.
10
Mutation versus repair: NEIL1 removal of hydantoin lesions in single-stranded, bulge, bubble, and duplex DNA contexts.突变与修复：NEIL1 在单链、凸起、泡状和双链 DNA 结构中去除乙内酰脲损伤。
Biochemistry. 2010 Mar 2;49(8):1658-66. doi: 10.1021/bi901852q.
引用本文的文献
1
Characterizing the excision of 7,8-dihydro-8-oxoadenine by thymine DNA glycosylase.胸腺嘧啶DNA糖基化酶对7,8-二氢-8-氧代腺嘌呤切除作用的表征
J Biol Chem. 2025 Jun 16;301(7):110363. doi: 10.1016/j.jbc.2025.110363.
2
Deprotonation of 8-Oxo-7,8-dihydroadenine Radical Cation in Free and Encumbered Context: A Theoretical Study.游离和受限环境中8-氧代-7,8-二氢腺嘌呤自由基阳离子的去质子化：一项理论研究
ACS Omega. 2024 Dec 13;9(51):50730-50741. doi: 10.1021/acsomega.4c08956. eCollection 2024 Dec 24.
3
8-Oxoadenine: A «New» Player of the Oxidative Stress in Mammals?8-氧代腺嘌呤：哺乳动物氧化应激中的一个“新”参与者？
Int J Mol Sci. 2024 Jan 22;25(2):1342. doi: 10.3390/ijms25021342.
4
NEIL1 Recoding due to RNA Editing Impacts Lesion-Specific Recognition and Excision.RNA 编辑导致的 NEIL1 重编码影响病变特异性识别和切除。
J Am Chem Soc. 2022 Aug 17;144(32):14578-14589. doi: 10.1021/jacs.2c03625. Epub 2022 Aug 2.
5
Insights into the mismatch discrimination mechanism of Y-family DNA polymerase Dpo4.Y 家族 DNA 聚合酶 Dpo4 错配识别机制的研究进展
Biochem J. 2021 May 14;478(9):1769-1781. doi: 10.1042/BCJ20210162.
6
Promutagenic bypass of 7,8-dihydro-8-oxoadenine by translesion synthesis DNA polymerase Dpo4.跨损伤合成 DNA 聚合酶 Dpo4 对 7,8-二氢-8-氧腺嘌呤的诱变旁路。
Biochem J. 2020 Aug 14;477(15):2859-2871. doi: 10.1042/BCJ20200449.
7
Mutagenesis mechanism of the major oxidative adenine lesion 7,8-dihydro-8-oxoadenine.主要氧化腺嘌呤损伤 7,8-二氢-8-氧腺嘌呤的诱变机制。
Nucleic Acids Res. 2020 May 21;48(9):5119-5134. doi: 10.1093/nar/gkaa193.
8
Petri net-based model of the human DNA base excision repair pathway.基于 Petri 网的人类 DNA 碱基切除修复途径模型。
PLoS One. 2019 Sep 13;14(9):e0217913. doi: 10.1371/journal.pone.0217913. eCollection 2019.
9
Mutagenic Replication of the Major Oxidative Adenine Lesion 7,8-Dihydro-8-oxoadenine by Human DNA Polymerases.人类 DNA 聚合酶对主要氧化腺嘌呤损伤 7,8-二氢-8-氧腺嘌呤的诱变复制。
J Am Chem Soc. 2019 Mar 20;141(11):4584-4596. doi: 10.1021/jacs.8b08551. Epub 2019 Mar 7.
10
DNA Repair Molecular Beacon assay: a platform for real-time functional analysis of cellular DNA repair capacity.DNA修复分子信标检测法：一种用于细胞DNA修复能力实时功能分析的平台。
Oncotarget. 2018 Aug 3;9(60):31719-31743. doi: 10.18632/oncotarget.25859.
本文引用的文献
1
Cockayne syndrome group B protein stimulates repair of formamidopyrimidines by NEIL1 DNA glycosylase.科凯恩综合征B组蛋白通过NEIL1 DNA糖基化酶刺激甲酰胺嘧啶的修复。
J Biol Chem. 2009 Apr 3;284(14):9270-9. doi: 10.1074/jbc.M807006200. Epub 2009 Jan 29.
2
Specificity of stimulation of human 8-oxoguanine-DNA glycosylase by AP endonuclease.AP核酸内切酶对人8-氧代鸟嘌呤-DNA糖基化酶刺激作用的特异性
Biochem Biophys Res Commun. 2008 Mar 28;368(1):175-9. doi: 10.1016/j.bbrc.2008.01.076. Epub 2008 Jan 24.
3
RNA polymerase II bypasses 8-oxoguanine in the presence of transcription elongation factor TFIIS.在转录延伸因子TFIIS存在的情况下，RNA聚合酶II绕过8-氧代鸟嘌呤。
DNA Repair (Amst). 2007 Jun 1;6(6):841-51. doi: 10.1016/j.dnarep.2007.01.014. Epub 2007 Mar 19.
4
Deoxyribophosphate lyase activity of mammalian endonuclease VIII-like proteins.哺乳动物类核酸内切酶 VIII 样蛋白的脱氧核糖磷酸裂解酶活性。
FEBS Lett. 2006 Sep 4;580(20):4916-22. doi: 10.1016/j.febslet.2006.08.011. Epub 2006 Aug 15.
5
Genetic effects of oxidative DNA damages: comparative mutagenesis of the imidazole ring-opened formamidopyrimidines (Fapy lesions) and 8-oxo-purines in simian kidney cells.氧化性DNA损伤的遗传效应：猴肾细胞中咪唑环开环的甲酰胺基嘧啶（Fapy损伤）和8-氧代嘌呤的比较诱变
Nucleic Acids Res. 2006 May 5;34(8):2305-15. doi: 10.1093/nar/gkl099. Print 2006.
6
DNA polymerase beta promotes recruitment of DNA ligase III alpha-XRCC1 to sites of base excision repair.DNA聚合酶β促进DNA连接酶IIIα-XRCC1募集至碱基切除修复位点。
Biochemistry. 2005 Aug 9;44(31):10613-9. doi: 10.1021/bi050085m.
7
Mouse NEIL1 protein is specific for excision of 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 4,6-diamino-5-formamidopyrimidine from oxidatively damaged DNA.小鼠NEIL1蛋白对从氧化损伤的DNA中切除2,6-二氨基-4-羟基-5-甲酰胺基嘧啶和4,6-二氨基-5-甲酰胺基嘧啶具有特异性。
Biochemistry. 2004 Dec 21;43(50):15909-14. doi: 10.1021/bi048162l.
8
Oxidative DNA damage and disease: induction, repair and significance.氧化性DNA损伤与疾病：诱导、修复及意义
Mutat Res. 2004 Sep;567(1):1-61. doi: 10.1016/j.mrrev.2003.11.001.
9
AP endonuclease-independent DNA base excision repair in human cells.人类细胞中不依赖脱嘌呤嘧啶内切酶的DNA碱基切除修复
Mol Cell. 2004 Jul 23;15(2):209-20. doi: 10.1016/j.molcel.2004.06.003.
10
Structural characterization of the Fpg family of DNA glycosylases.DNA糖基化酶Fpg家族的结构表征。
DNA Repair (Amst). 2003 Aug 12;2(8):839-62. doi: 10.1016/s1568-7864(03)00084-3.
Zotero 插件