在蛇形微通道中细胞的电动聚焦和过滤。
Electrokinetic focusing and filtration of cells in a serpentine microchannel.
出版信息
Biomicrofluidics. 2009 Nov 24;3(4):44109. doi: 10.1063/1.3267098.
Abstract
Focusing cells into a single stream is usually a necessary step prior to counting and separating them in microfluidic devices such as flow cytometers and cell sorters. This work presents a sheathless electrokinetic focusing of yeast cells in a planar serpentine microchannel using dc-biased ac electric fields. The concurrent pumping and focusing of yeast cells arise from the dc electrokinetic transport and the turn-induced acdc dielectrophoretic motion, respectively. The effects of electric field (including ac to dc field ratio and ac field frequency) and concentration (including buffer concentration and cell concentration) on the cell focusing performance were studied experimentally and numerically. A continuous electrokinetic filtration of E. coli cells from yeast cells was also demonstrated via their differential electrokinetic focusing in a serpentine microchannel.
摘要
将细胞聚焦成单一流通常是在微流控设备（如流式细胞仪和细胞分选器）中对其进行计数和分离之前的必要步骤。本工作提出了在平面蛇形微通道中使用直流偏置交流电场无鞘层电动聚焦酵母细胞。酵母细胞的同时泵送和聚焦分别源于直流电动传输和转弯诱导的 acdc 介电泳运动。实验和数值研究了电场（包括交流到直流场比和交流场频率）和浓度（包括缓冲液浓度和细胞浓度）对细胞聚焦性能的影响。还通过在蛇形微通道中对大肠杆菌细胞和酵母细胞进行差分电动聚焦，展示了从酵母细胞中连续电动过滤大肠杆菌细胞的方法。
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