在蛇形微通道中细胞的电动聚焦和过滤。
Electrokinetic focusing and filtration of cells in a serpentine microchannel.
出版信息
Biomicrofluidics. 2009 Nov 24;3(4):44109. doi: 10.1063/1.3267098.
Abstract
Focusing cells into a single stream is usually a necessary step prior to counting and separating them in microfluidic devices such as flow cytometers and cell sorters. This work presents a sheathless electrokinetic focusing of yeast cells in a planar serpentine microchannel using dc-biased ac electric fields. The concurrent pumping and focusing of yeast cells arise from the dc electrokinetic transport and the turn-induced acdc dielectrophoretic motion, respectively. The effects of electric field (including ac to dc field ratio and ac field frequency) and concentration (including buffer concentration and cell concentration) on the cell focusing performance were studied experimentally and numerically. A continuous electrokinetic filtration of E. coli cells from yeast cells was also demonstrated via their differential electrokinetic focusing in a serpentine microchannel.
摘要
将细胞聚焦成单一流通常是在微流控设备（如流式细胞仪和细胞分选器）中对其进行计数和分离之前的必要步骤。本工作提出了在平面蛇形微通道中使用直流偏置交流电场无鞘层电动聚焦酵母细胞。酵母细胞的同时泵送和聚焦分别源于直流电动传输和转弯诱导的 acdc 介电泳运动。实验和数值研究了电场（包括交流到直流场比和交流场频率）和浓度（包括缓冲液浓度和细胞浓度）对细胞聚焦性能的影响。还通过在蛇形微通道中对大肠杆菌细胞和酵母细胞进行差分电动聚焦，展示了从酵母细胞中连续电动过滤大肠杆菌细胞的方法。
相似文献
1
Electrokinetic focusing and filtration of cells in a serpentine microchannel.在蛇形微通道中细胞的电动聚焦和过滤。
Biomicrofluidics. 2009 Nov 24;3(4):44109. doi: 10.1063/1.3267098.
2
Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels.棘轮微通道中粒子和细胞的被动介电泳聚焦
Micromachines (Basel). 2020 Apr 25;11(5):451. doi: 10.3390/mi11050451.
3
Dielectrophoretic focusing of particles in a microchannel constriction using DC-biased AC flectric fields.使用直流偏置交流电场在微通道缩颈中对粒子进行介电泳聚焦。
Electrophoresis. 2009 Aug;30(15):2668-75. doi: 10.1002/elps.200900017.
4
AC Insulator-Based Dielectrophoretic Focusing of Particles and Cells in an "Infinite" Microchannel.基于 AC 绝缘子的粒子和细胞在“无限”微通道中的介电泳聚焦。
Anal Chem. 2021 Apr 13;93(14):5947-5953. doi: 10.1021/acs.analchem.1c00697. Epub 2021 Apr 1.
5
Electrokinetic particle entry into microchannels.电动颗粒进入微通道。
Electrophoresis. 2012 Mar;33(6):916-22. doi: 10.1002/elps.201100484.
6
Negative dielectrophoresis-based particle separation by size in a serpentine microchannel.基于负介电泳的蛇形微通道中按粒径分离颗粒。
Electrophoresis. 2011 Feb;32(5):527-31. doi: 10.1002/elps.201000396. Epub 2011 Feb 3.
7
dc electrokinetic transport of cylindrical cells in straight microchannels.直微通道中圆柱形细胞的直流电动传输。
Biomicrofluidics. 2009 Nov 24;3(4):44110. doi: 10.1063/1.3267095.
8
Fluid Elasticity-Enhanced Insulator-Based Dielectrophoresis for Sheath-Free Particle Focusing in Very Dilute Polymer Solutions.用于在极稀聚合物溶液中实现无鞘层粒子聚焦的基于流体弹性增强绝缘体的介电泳
Anal Chem. 2023 Oct 31;95(43):16013-16020. doi: 10.1021/acs.analchem.3c03311. Epub 2023 Oct 19.
9
Continuous dielectrophoretic separation of particles in a spiral microchannel.在螺旋微通道中连续介电泳分离颗粒。
Electrophoresis. 2010 Apr;31(8):1382-8. doi: 10.1002/elps.200900736.
10
Focusing particles by induced charge electrokinetic flow in a microchannel.通过微通道中的感应电荷电动流聚焦粒子。
Electrophoresis. 2016 Feb;37(4):666-75. doi: 10.1002/elps.201500361.
引用本文的文献
1
Development of a DC-Biased AC-Stimulated Microfluidic Device for the Electrokinetic Separation of Bacterial and Yeast Cells.基于直流偏置交流激励的微流控芯片用于细菌和酵母细胞的电动分离。
Biosensors (Basel). 2024 May 9;14(5):237. doi: 10.3390/bios14050237.
2
Assessing the Discriminatory Capabilities of iEK Devices under DC and DC-Biased AC Stimulation Potentials.评估iEK设备在直流和直流偏置交流刺激电位下的辨别能力。
Micromachines (Basel). 2023 Dec 14;14(12):2239. doi: 10.3390/mi14122239.
3
The latest advances on nonlinear insulator-based electrokinetic microsystems under direct current and low-frequency alternating current fields: a review.直流和低频交流场下基于非线性绝缘子的电动微系统的最新进展：综述。
Anal Bioanal Chem. 2022 Jan;414(2):885-905. doi: 10.1007/s00216-021-03687-9. Epub 2021 Oct 19.
4
Charge-Based Separation of Micro- and Nanoparticles.基于电荷的微米和纳米颗粒分离
Micromachines (Basel). 2020 Nov 18;11(11):1014. doi: 10.3390/mi11111014.
5
Passive Dielectrophoretic Focusing of Particles and Cells in Ratchet Microchannels.棘轮微通道中粒子和细胞的被动介电泳聚焦
Micromachines (Basel). 2020 Apr 25;11(5):451. doi: 10.3390/mi11050451.
6
Enhanced Throughput for Electrokinetic Manipulation of Particles and Cells in a Stacked Microfluidic Device.用于堆叠式微流控装置中粒子和细胞电动操控的增强通量
Micromachines (Basel). 2016 Sep 1;7(9):156. doi: 10.3390/mi7090156.
7
Sheathless electrokinetic particle separation in a bifurcating microchannel.分叉微通道中的无鞘电动粒子分离
Biomicrofluidics. 2016 Sep 16;10(5):054104. doi: 10.1063/1.4962875. eCollection 2016 Sep.
8
A Microflow Cytometer with a Rectangular Quasi-Flat-Top Laser Spot.一种具有矩形准平顶激光光斑的微流控细胞仪。
Sensors (Basel). 2016 Sep 11;16(9):1474. doi: 10.3390/s16091474.
9
On utilizing alternating current-flow field effect transistor for flexibly manipulating particles in microfluidics and nanofluidics.关于利用交流场效应晶体管在微流体和纳米流体中灵活操控粒子。
Biomicrofluidics. 2016 May 12;10(3):034105. doi: 10.1063/1.4949771. eCollection 2016 May.
10
Microfluidic electrical sorting of particles based on shape in a spiral microchannel.基于螺旋微通道中形状的微流控电粒子分选。
Biomicrofluidics. 2014 Jan 14;8(1):014101. doi: 10.1063/1.4862355. eCollection 2014 Jan.
本文引用的文献
1
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).聚二甲基硅氧烷微流控系统的快速成型
Anal Chem. 1998 Dec 1;70(23):4974-84. doi: 10.1021/ac980656z.
2
Particle electrophoresis and dielectrophoresis in curved microchannels.在弯曲微通道中的粒子电泳和介电泳。
J Colloid Interface Sci. 2009 Dec 15;340(2):285-90. doi: 10.1016/j.jcis.2009.08.031. Epub 2009 Aug 23.
3
Vertical hydrodynamic focusing in glass microchannels.玻璃微通道中的垂直流体动力学聚焦。
Biomicrofluidics. 2009 Jan 8;3(1):14101. doi: 10.1063/1.3055278.
4
Dielectrophoretic manipulation of particles in a modified microfluidic H filter with multi-insulating blocks.在具有多个绝缘块的改进型微流控H过滤器中对颗粒进行介电泳操纵。
Biomicrofluidics. 2008 Aug 11;2(3):34105. doi: 10.1063/1.2973661.
5
Dielectrophoretic focusing of particles in a microchannel constriction using DC-biased AC flectric fields.使用直流偏置交流电场在微通道缩颈中对粒子进行介电泳聚焦。
Electrophoresis. 2009 Aug;30(15):2668-75. doi: 10.1002/elps.200900017.
6
Optical gradient flow focusing.光学梯度流聚焦
Opt Express. 2007 May 14;15(10):6167-76. doi: 10.1364/oe.15.006167.
7
A three-dimensional (3D) particle focusing channel using the positive dielectrophoresis (pDEP) guided by a dielectric structure between two planar electrodes.一种三维（3D）粒子聚焦通道，其利用由两个平面电极之间的介电结构引导的正介电泳（pDEP）。
Lab Chip. 2009 Mar 7;9(5):686-91. doi: 10.1039/b812213j. Epub 2008 Nov 24.
8
Sheathless focusing of microbeads and blood cells based on hydrophoresis.基于流体电泳的无鞘微珠和血细胞聚焦
Small. 2008 May;4(5):634-41. doi: 10.1002/smll.200700308.
9
Sheathless hydrophoretic particle focusing in a microchannel with exponentially increasing obstacle arrays.在具有指数增长障碍物阵列的微通道中进行无鞘流体动力粒子聚焦。
Anal Chem. 2008 Apr 15;80(8):3035-9. doi: 10.1021/ac8001319. Epub 2008 Mar 21.
10
Zeta potential and electroosmotic mobility in microfluidic devices fabricated from hydrophobic polymers: 2. Slip and interfacial water structure.由疏水性聚合物制成的微流控装置中的zeta电位和电渗迁移率：2. 滑移和界面水结构。
Electrophoresis. 2008 Mar;29(5):1102-14. doi: 10.1002/elps.200800735.
Zotero 插件