Division of Cancer Chemotherapy, Miyagi Cancer Center Research Institute, 47-1 Nodayama, Medeshima-Shiode, Natori, Miyagi, 981-1293, Japan.
J Neurooncol. 2010 Oct;100(1):43-9. doi: 10.1007/s11060-010-0147-3. Epub 2010 Mar 10.
Cell division cycle 25 (CDC25) phosphatases are cell-cycle regulatory proteins which are overexpressed in a significant number of human cancers. This study evaluated the role of CDC25 phosphatases in human glioma proliferation. Upregulation of CDC25A was observed in human glioma specimens and human glioma cell lines. Comparison of expression levels of CDC25A and CDC25B messenger ribonucleic acid (RNA) to Ki-67 labeling index in glioma tissues found that Ki-67 labeling index was significantly correlated with the expression of CDC25A, but not with that of CDC25B. Depletion of CDC25A by small interfering RNA and inhibition of CDC25 suppressed cell proliferation and induced apoptosis in glioma cell lines, indicating that CDC25A is a potential target for the development of new therapy for glioma.
细胞分裂周期蛋白 25（CDC25）磷酸酶是细胞周期调节蛋白，在大量人类癌症中过度表达。本研究评估了 CDC25 磷酸酶在人类神经胶质瘤增殖中的作用。在人类神经胶质瘤标本和神经胶质瘤细胞系中观察到 CDC25A 的上调。比较 CDC25A 和 CDC25B 信使核糖核酸（RNA）在胶质瘤组织中的表达水平与 Ki-67 标记指数发现，Ki-67 标记指数与 CDC25A 的表达显著相关，但与 CDC25B 的表达无关。小干扰 RNA 敲低 CDC25A 和抑制 CDC25 抑制了神经胶质瘤细胞系的增殖并诱导了细胞凋亡，表明 CDC25A 是开发新的神经胶质瘤治疗方法的潜在靶点。
