Chan Juliana M, Valencia Pedro M, Zhang Liangfang, Langer Robert, Farokhzad Omid C
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Methods Mol Biol. 2010;624:163-75. doi: 10.1007/978-1-60761-609-2_11.
The use of biodegradable polymeric nanoparticles (NPs) for controlled drug delivery has shown significant therapeutic potential. Concurrently, targeted delivery technologies are becoming increasingly important as a scientific area of investigation. In cancer, targeted polymeric NPs can be used to deliver chemotherapies to tumor cells with greater efficacy and reduced cytotoxicity on peripheral healthy tissues. In this chapter, we describe the methods of (1) preparation and characterization of drug-encapsulated polymeric NPs formulated with biocompatible and biodegradable poly(D,L-lactic-co-glycolic acid)-poly(ethylene glycol) (PLGA-b-PEG) copolymers; (2) surface functionalization of the polymeric NPs with the A10 2'-fluoropyrimidine ribonucleic acid (RNA) aptamers that recognize the prostate-specific membrane antigen (PSMA) on prostate cancer cells; and (3) evaluation of the binding properties of these targeted polymeric NPs to PSMA-expressing prostate cancer cells in vitro and in vivo. These methods may contribute to the development of other useful polymeric NPs to deliver a spectrum of chemotherapeutic, diagnostic, and imaging agents for various applications.
使用可生物降解的聚合物纳米颗粒（NPs）进行药物控释已显示出巨大的治疗潜力。与此同时，作为一个科学研究领域，靶向递送技术正变得越来越重要。在癌症治疗中，靶向聚合物纳米颗粒可用于将化疗药物递送至肿瘤细胞，从而提高疗效并降低对周围健康组织的细胞毒性。在本章中，我们描述了以下方法：（1）制备和表征用生物相容性和可生物降解的聚（D，L-乳酸-共-乙醇酸）-聚（乙二醇）（PLGA-b-PEG）共聚物配制的载药聚合物纳米颗粒；（2）用识别前列腺癌细胞上前列腺特异性膜抗原（PSMA）的A10 2'-氟嘧啶核糖核酸（RNA）适体对聚合物纳米颗粒进行表面功能化；（3）评估这些靶向聚合物纳米颗粒在体外和体内与表达PSMA的前列腺癌细胞的结合特性。这些方法可能有助于开发其他有用的聚合物纳米颗粒，以递送一系列用于各种应用的化疗、诊断和成像剂。
