Department of Oromaxillofacial Regeneration, Osaka University, Suita, Japan.
Tissue Eng Part A. 2010 Aug;16(8):2497-504. doi: 10.1089/ten.TEA.2009.0523.
A morphologically controlled three-dimensional (3D) cell construct composed of only cells and having no scaffold material might be a valuable biologic material for tissue engineering applications, as the scaffold materials can cause delay of tissue regeneration in some conditions. To obtain such a 3D cell construct, a 3D thermoresponsive hydrogel (poly-N-isopropylacrylamide) was prepared as a mold material that changes its volume depending on the temperature. Three-dimensional osteoblast cell constructs with a variety of morphologies as well as a monolayered cell sheet were obtained by decreasing the surrounding temperature of the hydrogel designed with a predefined shape and formed by curing in a polymer mold manufactured via 3D printing. The cell sheet or 3D cell constructs detachment resulted from a simple change in the gel volume, not by the surface chemistry of the gel, because the surface hydrophilicity of the gel was maintained over a wide temperature range. These 2D/3D cell constructs have numbers of exciting applications such as cell carriers for tissue regeneration or as model tissues for the biological study.
一种仅由细胞组成且不含支架材料的形态可控三维（3D）细胞构建体可能是组织工程应用的有价值的生物材料，因为在某些情况下支架材料会延迟组织再生。为了获得这样的 3D 细胞构建体，制备了一种形态响应性水凝胶（聚 N-异丙基丙烯酰胺）作为模具材料，其体积会根据温度而变化。通过降低预先设计形状的水凝胶的周围温度，并在通过 3D 打印制造的聚合物模具中固化，获得具有各种形态的 3D 成骨细胞构建体以及单层细胞片。细胞片或 3D 细胞构建体的脱落是由于凝胶体积的简单变化引起的，而不是由于凝胶的表面化学性质引起的，因为凝胶的表面亲水性在很宽的温度范围内得以保持。这些 2D/3D 细胞构建体具有许多令人兴奋的应用，例如组织再生的细胞载体或用于生物学研究的模型组织。
