Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
J Neurochem. 2010 Jun;113(6):1436-46. doi: 10.1111/j.1471-4159.2010.06679.x. Epub 2010 Mar 10.
It is generally believed that temporary moderate stress to a living organism has protective and adaptive effects, but little is known about the responses of CNS to the moderate stresses at molecular level. This study aims to investigate the gene expression changes induced by moderate stress in CNS stress- and nociception-related regions of rats. Moderate restraint was applied to rats for 50 min and cDNA microarrays were used to detect the differential gene expression in different CNS regions. Transcriptome profiling analysis showed that at acute stage stress-related genes were up-regulated in arcuate nucleus; fight-or-flight behavior-related genes were up-regulated in periaqueductal gray, while nitric oxide and GABA signal transmission-related genes were up-regulated in spinal dorsal horn. In addition, immune-related genes were broadly regulated, especially at the late stage. These results suggested that specific genes of certain gene ontology categories were spatiotemporally regulated in specific CNS regions related to relevant functions under moderate external stimuli at acute stage, while immune response was broadly regulated at the late stage. The co-regulated genes among the three different CNS regions may play general roles in CNS when exposed to moderate stress. Furthermore, these results will help to elucidate the physiological processes involved in moderate stress in CNS.
人们普遍认为，生活在生物体的暂时适度的压力具有保护和适应性的影响，但对中枢神经系统的适度压力的分子水平的反应知之甚少。本研究旨在探讨中度应激诱导的中枢神经系统应激和痛觉相关区域的大鼠基因表达变化。中度束缚应用于大鼠 50 分钟和 cDNA 微阵列用于检测不同中枢神经系统区域的差异基因表达。转录组分析表明，在急性应激相关基因在弓状核上调;战斗或逃跑行为相关基因在上丘上调，而一氧化氮和 GABA 信号转导相关基因在脊髓背角上调。此外，免疫相关基因广泛调控，尤其是在晚期。这些结果表明，在急性阶段，特定的基因本体类别特定的基因在特定的与相关功能有关的中枢神经系统区域时空调节，而在晚期，免疫反应广泛调控。三个不同的中枢神经系统区域之间的共同调控基因可能在中枢神经系统中发挥一般作用时，暴露于中度压力。此外，这些结果将有助于阐明在中枢神经系统中涉及的生理过程中度应激。
