Department of Civil and Environmental Engineering, Clarkson University, Potsdam, NY 13699-5710, USA.
Water Res. 2010 May;44(9):2793-802. doi: 10.1016/j.watres.2010.02.012. Epub 2010 Feb 19.
Extensive research has been done to characterize transport of bacteria in porous media; however, little is understood on how the presence of non-aqueous phase liquids (NAPLs) coupled with the growth state and carbon source of bacteria affect bacterial transport. The objective of this research is to quantify the bacterial adhesion of Pseudomonas saccharophilia P15 (P15), which is known to biodegrade polycyclic aromatic hydrocarbons (PAH) and to interact with coal tars, within a NAPL-water-mineral system. Through a series of short-pulse column experiments, the transport and deposition of P15 in porous media (quartz sand) as a function of growth state and carbon sources (peptone and naphthalene), and in the presence and absence of residual NAPL (hexadecane), is measured and evaluated. Coating 20% of the quartz grain with hexadecane as a model NAPL increased the retention of P15 by as much as a factor of 26 as compared to the retention exhibited in quartz sand with no NAPL present. P15 grown on peptone and in the late exponential growth state exhibited a greater amount of deposition within the hexadecane column than when it was grown on naphthalene or was in early exponential growth phase. During early growth stage P15 grown on naphthalene adhered stronger to the porous media compared to when grown on peptone. Results were compared with results of MATH assays, where P15 partitioning to hexadecane was evaluated as a function of carbon source and growth state.
已经进行了广泛的研究来描述细菌在多孔介质中的迁移，但对于非水相液体（NAPL）的存在如何影响细菌迁移，以及细菌的生长状态和碳源如何影响细菌迁移，人们知之甚少。本研究的目的是量化已知可生物降解多环芳烃（PAH）并与煤焦油相互作用的嗜糖假单胞菌 P15（P15）的细菌附着，该研究是在 NAPL-水-矿物系统中进行的。通过一系列短脉冲柱实验，测量和评估了 P15 在多孔介质（石英砂）中的迁移和沉积，作为生长状态和碳源（蛋白胨和萘）的函数，以及在存在和不存在残余 NAPL（十六烷）的情况下。将 20%的石英砂用十六烷作为模型 NAPL 涂覆，与没有 NAPL 存在的石英砂相比，P15 的保留率增加了多达 26 倍。在十六烷柱中，与在蛋白胨上生长或处于早期指数生长阶段相比，在萘上生长且处于晚期指数生长阶段的 P15 有更多的沉积。在早期生长阶段，与在蛋白胨上生长相比，在萘上生长的 P15 对多孔介质的附着更强。结果与 MATH 测定的结果进行了比较，其中评估了 P15 对十六烷的分配作为碳源和生长状态的函数。
