Li Jiuyi, Zhao Xiaokang, Tian Xiujun, Li Jin, Sjollema Jelmer, Wang Aimin
School of Civil Engineering, Beijing Jiaotong University, Beijing, China.
Department of Biomedical Engineering, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Appl Environ Microbiol. 2015 Mar;81(6):2199-205. doi: 10.1128/AEM.03740-14. Epub 2015 Jan 16.
The fate and transport of pathogenic bacteria from wastewater treatment facilities in the Earth's subsurface have attracted extensive concern over recent decades, while the impact of treated-wastewater chemistry on bacterial viability and transport behavior remains unclear. The influence of retention time in effluent from a full-scale municipal wastewater treatment plant on the survival and deposition of Staphylococcus aureus and Escherichia coli strains in sand columns was investigated in this paper. In comparison to the bacteria cultivated in nutrient-rich growth media, retention in treated wastewater significantly reduced the viability of all strains. Bacterial surface properties, e.g., zeta potential, hydrophobicity, and surface charges, varied dramatically in treated wastewater, though no universal trend was found for different strains. Retention in treated wastewater effluent resulted in changes in bacterial deposition in sand columns. Longer retention periods in treated wastewater decreased bacterial deposition rates for the strains evaluated and elevated the transport potential in sand columns. We suggest that the wastewater quality should be taken into account in estimating the fate of pathogenic bacteria discharged from wastewater treatment facilities and the risks they pose in the aquatic environment.
近几十年来，地球地下污水处理设施中病原菌的归宿和迁移受到了广泛关注，而经处理废水的化学性质对细菌生存能力和迁移行为的影响仍不明确。本文研究了全尺寸城市污水处理厂出水的停留时间对金黄色葡萄球菌和大肠杆菌菌株在砂柱中存活和沉积的影响。与在营养丰富的生长培养基中培养的细菌相比，在经处理的废水中停留显著降低了所有菌株的生存能力。细菌表面性质，如ζ电位、疏水性和表面电荷，在经处理的废水中有显著变化，尽管不同菌株未发现普遍趋势。在经处理的废水流出物中停留导致砂柱中细菌沉积发生变化。在经处理的废水中较长的停留时间降低了所评估菌株的细菌沉积速率，并提高了在砂柱中的迁移潜力。我们建议，在评估污水处理设施排放的病原菌归宿及其在水生环境中造成的风险时，应考虑废水质量。
