Department of Chemical Engineering and Environmental Technology, University of Valladolid, 47011 Valladolid, Spain.
Bioresour Technol. 2010 Jul;101(14):5150-8. doi: 10.1016/j.biortech.2010.02.010. Epub 2010 Mar 9.
Two green microalgae (Scenedesmus obliquus and Chlorella sorokiniana), one cyanobacterium (Spirulina platensis), one euglenophyt (Euglena viridis) and two microalgae consortia were evaluated for their ability to support carbon, nitrogen and phosphorous removal in symbiosis with activated sludge bacteria during the biodegradation of four and eight times diluted piggery wastewater in batch tests. C. sorokiniana and E. viridis were capable of supporting the biodegradation of four and eight times diluted wastewater. On the other hand, while S. obliquus and the consortia isolated from a swine manure stabilization pond were only able to grow in eight times diluted wastewater, S. platensis and the consortium isolated from a high rate algal pond treating swine manure were totally inhibited regardless of the dilution applied. TOC removal efficiencies (RE) ranging from 42% to 55% and NH(4)(+)-RE from 21% to 39% were recorded in the tests exhibiting photosynthetic oxygenation. The similar oxygen production rates exhibited by the tested microalgae under autotrophic conditions (from 116 to 133mgO(2)L(-1)d(-1)) suggested that factors other than the photosynthetic oxygenation potential governed piggery wastewater biodegradation. Microalgal tolerance towards NH(3) was hypothesized as the key selection criterion. Further studies in a continuous algal-bacterial photobioreactor inoculated with C. sorokiniana, S. obliquus and S. platensis showed that C. sorokiniana, the species showing the highest NH(3)-tolerance, rapidly outcompeted the rest of the microalgae during the biodegradation of eight times diluted wastewater, achieving TOC and NH(4)(+)-RE comparable to those recorded in the batch biodegradation tests.
两种绿色微藻（斜生栅藻和普通小球藻）、一种蓝藻（钝顶螺旋藻）、一种绿眼虫（绿眼虫）和两种微藻共生体在批式试验中，评估了它们在与活性污泥细菌共生时，从 4 倍和 8 倍稀释猪粪废水中去除碳、氮和磷的能力。C. sorokiniana 和 E. viridis 能够支持 4 倍和 8 倍稀释废水的生物降解。另一方面，虽然 S. obliquus 和从猪粪稳定塘中分离的共生体只能在 8 倍稀释废水中生长，但 S. platensis 和从处理猪粪的高速藻类塘中分离的共生体无论应用何种稀释度，均完全受到抑制。在表现出光合作用增氧的试验中，记录到 TOC 去除效率（RE）为 42%至 55%，NH（4）（+）-RE 为 21%至 39%。在自养条件下，测试的微藻表现出相似的产氧率（从 116 到 133mgO（2）L（-1）d（-1）），这表明除了光合作用增氧潜力之外，还有其他因素控制着猪粪废水的生物降解。微藻对 NH（3）的耐受性被假设为关键选择标准。在连续的藻类-细菌光生物反应器中接种 C. sorokiniana、S. obliquus 和 S. platensis 的进一步研究表明，在 8 倍稀释废水的生物降解过程中，表现出最高 NH（3）-耐受性的 C. sorokiniana 迅速淘汰了其余的微藻，实现了与批式生物降解试验中记录的 TOC 和 NH（4）（+）-RE 相当的去除效率。
