脑肿瘤大鼠胸腺细胞与脾细胞中淋巴因子激活的杀伤活性比较。
Comparison of lymphokine-activated killer activities between thymocytes and splenocytes in rats with brain tumors.
作者信息
Matsuura H, Imaya H
机构信息
Neurosurgery Service, Saitama Neurosurgical Institute, Kohnosu, Japan.
出版信息
Cancer Immunol Immunother. 1991;33(1):50-3. doi: 10.1007/BF01742528.
We studied the lymphokine-activated killer (LAK) activity in splenocytes and thymocytes of rats with brain tumors chronologically from the early stage to the late stage, in order to clarify how much LAK activity would be developed at each stage. Simultaneously the natural killer (NK) activity in splenocytes, as one aspect of the host immunocompetence, was also determined. The splenic NK activity was significantly depressed in rats with brain tumors during the 2nd and 3rd weeks after tumor transplantation, as compared with normal controls. On the other hand, the splenocytes incubated with interleukin-2 showed the same killer activity in rats with brain tumors as in normal rats at all times. The LAK activity in thymocytes from rats with brain tumors was significantly higher than that of controls in the 1st and 2nd weeks and became equal to that of the controls during the 3rd week. The killer activity after incubation with interleukin-2 in thymocytes was superior to that in splenocytes throughout the experiment in both tumor-bearing rats and controls, which suggested that the precursor of LAK cells was not NK cells.
我们按时间顺序研究了脑肿瘤大鼠从早期到晚期脾细胞和胸腺细胞中的淋巴因子激活的杀伤细胞（LAK）活性，以阐明每个阶段会产生多少LAK活性。同时，作为宿主免疫能力的一个方面，还测定了脾细胞中的自然杀伤（NK）活性。与正常对照组相比，肿瘤移植后第2周和第3周，脑肿瘤大鼠的脾脏NK活性显著降低。另一方面，用白细胞介素-2孵育的脾细胞在脑肿瘤大鼠中始终表现出与正常大鼠相同的杀伤活性。脑肿瘤大鼠胸腺细胞中的LAK活性在第1周和第2周显著高于对照组，在第3周时与对照组相等。在整个实验过程中，无论是荷瘤大鼠还是对照组，胸腺细胞经白细胞介素-2孵育后的杀伤活性均优于脾细胞，这表明LAK细胞的前体不是NK细胞。
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