Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.
Mol Phylogenet Evol. 2010 Jul;56(1):441-50. doi: 10.1016/j.ympev.2010.02.025. Epub 2010 Feb 26.
The genus Erophaca comprises a single herbaceous perennial species with two subspecies distributed at opposite ends of the Mediterranean region. We used nrDNA ITS to investigate the phylogeny of the genus, and AFLP markers (9 primers, 20 populations) to establish the genetic relationship between subspecies, and among populations at each side of the Gibraltar Strait. According to nrDNA ITS, Erophaca is monophyletic, old (Miocene), and sister to the Astragalean clade. Life form attributes and molecular clock estimates suggest that Erophaca is one of the many Tertiary relicts that form part of the present Mediterranean flora. Within the occidental subspecies, European plants are clearly derived from North-African populations (Morocco) which, despite being rare on a regional scale, present the highest genetic diversity (as estimated by private and rare fragment numbers). In general, genetic diversity decreased with increasing distance from Morocco. AFLP and nrDNA ITS markers evidenced that the Eastern and the Western subspecies are genetically distinct. Possible causes for their disjunct distribution are discussed.
该属由一个单一的草本多年生物种组成，有两个亚种分布在地中海地区的两端。我们使用 nrDNA ITS 来研究属的系统发育，并用 AFLP 标记（9 个引物，20 个种群）来建立亚种之间以及直布罗陀海峡两侧种群之间的遗传关系。根据 nrDNA ITS，Erophaca 是单系的，古老的（中新世），并且与 Astragalean 分支是姐妹关系。生活形式特征和分子钟估计表明，Erophaca 是许多第三纪遗迹之一，构成了现代地中海植物区系的一部分。在西方亚种中，欧洲植物显然是从北非种群（摩洛哥）衍生而来的（尽管在区域尺度上很罕见，但摩洛哥种群具有最高的遗传多样性（根据私有和稀有片段数量估计）。一般来说，遗传多样性随着与摩洛哥距离的增加而降低。AFLP 和 nrDNA ITS 标记表明，东部和西部亚种在遗传上是不同的。讨论了它们间断分布的可能原因。
