Departments of Obstetrics and Gynecology, and Physiology and Biophysics, Virginia Commonwealth University Medical Center, Richmond, Virginia 23298, USA.
Reprod Sci. 2010 May;17(5):465-70. doi: 10.1177/1933719110361960. Epub 2010 Mar 10.
The objective of this study was to determine whether (1) neutrophils of preeclamptic women express cyclooxygenase 2 (COX-2) and (2) COX-2 mediates neutrophil interleukin 8 (IL-8) production. Neutrophils were isolated from heparinized blood collected from women with preeclampsia and normal pregnancy and from normal nonpregnant women. Cyclooxygenase 2 protein was measured in the neutrophils of the 3 groups by specific enzyme-linked immunosorbent assay (ELISA). To study the role of COX-2 in IL-8 production, neutrophils of normal pregnant women were activated with arachidonic acid (AA) and incubated overnight in Medium 199 with and without NS398, a specific COX-2 inhibitor. We found that neutrophils from preeclamptic women expressed significantly more COX-2 than neutrophils from normal pregnant or normal nonpregnant women. Arachidonic acid activated neutrophils to produce IL-8, which was completely inhibited by NS398. These data demonstrate that COX-2 is expressed in neutrophils of preeclamptic women and that COX-2 mediates IL-8 production by activated neutrophils.
本研究旨在确定（1）子痫前期妇女的中性粒细胞是否表达环氧化酶 2（COX-2），以及（2）COX-2 是否介导中性粒细胞白细胞介素 8（IL-8）的产生。从肝素化血液中分离出子痫前期和正常妊娠妇女以及正常非妊娠妇女的中性粒细胞。通过特定的酶联免疫吸附测定（ELISA）测量 3 组中性粒细胞中的 COX-2 蛋白。为了研究 COX-2 在 IL-8 产生中的作用，用花生四烯酸（AA）激活正常妊娠妇女的中性粒细胞，并在含和不含 NS398（一种特定的 COX-2 抑制剂）的 Medium 199 中孵育过夜。我们发现，子痫前期妇女的中性粒细胞表达的 COX-2 明显多于正常妊娠或正常非妊娠妇女的中性粒细胞。花生四烯酸激活中性粒细胞产生白细胞介素 8，而 NS398 可完全抑制白细胞介素 8 的产生。这些数据表明，COX-2 存在于子痫前期妇女的中性粒细胞中，并且 COX-2 介导激活的中性粒细胞中白细胞介素 8 的产生。
