Laboratorio de Canales iónicos-Neurofisiología, Instituto de Investigaciones Médicas A. Lanari, Universidad de Buenos Aires-CONICET, Argentina.
Reprod Sci. 2010 May;17(5):444-53. doi: 10.1177/1933719110361385. Epub 2010 Mar 10.
Trophoblastic abnormalities have a central role in the pathophysiology of preeclampsia, and some placental hormones, such as human chorionic gonadotropin (hCG), could affect the placental function. Here, we hypothesized that the elevated serum levels of hCG may be involved in the increased aquaporin-9 (AQP9) protein expression in preeclamptic placentas via adenosine 3('),5(')-cyclic phosphate (cAMP) pathways. Normal placental explants were cultured with different concentrations of recombinant hCG or 8-Br-cAMP, a potent analogue of cAMP. We evaluated AQP9 protein expression and localization. After both treatments, we localized AQP9 in the apical membrane of syncytiotrophoblast and in the cytoplasm. We also observed a concentration-dependent effect on AQP9 protein expression. In addition, water uptake increased 1.6-fold in explants treated with hCG. Our results suggest that hCG may increase AQP9 protein expression and functionality via cAMP pathways. Although, in preeclamptic placentas high levels of hCG may upregulate AQP9 protein expression, AQP9 functionality was reduced possibly by other factors.
滋养层异常在子痫前期的病理生理学中起着核心作用，一些胎盘激素，如人绒毛膜促性腺激素（hCG），可能影响胎盘功能。在这里，我们假设升高的血清 hCG 水平可能通过腺苷酸 3'（5'）-环磷酸（cAMP）途径参与子痫前期胎盘中水通道蛋白-9（AQP9）蛋白表达的增加。用不同浓度的重组 hCG 或 cAMP 的有效类似物 8-Br-cAMP 培养正常胎盘外植体。我们评估了 AQP9 蛋白表达和定位。在两种处理后，我们观察到 AQP9 在合体滋养层的顶膜和细胞质中的定位。我们还观察到对 AQP9 蛋白表达的浓度依赖性影响。此外，用 hCG 处理的外植体中水的摄取增加了 1.6 倍。我们的结果表明，hCG 可能通过 cAMP 途径增加 AQP9 蛋白表达和功能。尽管在子痫前期胎盘中高水平的 hCG 可能上调 AQP9 蛋白表达，但 AQP9 功能可能被其他因素降低。
