Biomedical Photonic Imaging Group, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Nanotechnology. 2010 Apr 9;21(14):145101. doi: 10.1088/0957-4484/21/14/145101. Epub 2010 Mar 11.
We evaluated cellular responses to polymer-treated gold nanorods, which were synthesized using the standard wet-chemistry method that utilizes hexadecyltrimethylammonium bromide (CTAB). The nanorod dispersions were coated with either polystyrene sulfonate (PSS) or polyethylene glycol (PEG). Two sizes of nanorods were tested, with optical responses peaking at 628 and 773 nm. The cells were from mammary adenocarcinoma (SKBR3), Chinese Hamster Ovary (CHO), mouse myoblast (C2C12) and Human Leukemia (HL60) cell lines. Their mitochondrial function following exposure to the nanorods were assessed using the MTS assay. We found PEGylated particles to have superior biocompatibility compared with PSS-coated nanorods, which showed substantial cytotoxicity. Electron microscopy showed no cellular uptake of PEGylated particles compared with their PSS counterparts. PEGylated gold nanorods also exhibited better dispersion stability in the presence of cell growth medium; PSS-coated rods tended to flocculate or cluster. In the case of the PSS particles, toxicity correlated with surface area across the two sizes of nanorods studied.
我们评估了聚合物处理的金纳米棒的细胞反应，这些纳米棒是使用标准的湿化学方法合成的，该方法利用十六烷基三甲基溴化铵（CTAB）。纳米棒分散体涂有聚苯乙烯磺酸盐（PSS）或聚乙二醇（PEG）。测试了两种尺寸的纳米棒，其光学响应在 628 和 773nm 处达到峰值。细胞来自乳腺腺癌（SKBR3）、中国仓鼠卵巢（CHO）、小鼠成肌细胞（C2C12）和人白血病（HL60）细胞系。使用 MTS 测定法评估了它们暴露于纳米棒后的线粒体功能。我们发现与 PSS 涂层纳米棒相比，PEG 涂层颗粒具有更好的生物相容性，PSS 涂层纳米棒显示出明显的细胞毒性。电子显微镜显示，与 PSS 对应物相比，细胞内没有摄取 PEG 颗粒。在细胞生长培养基存在的情况下，PEG 修饰的金纳米棒也表现出更好的分散稳定性；PSS 涂层的棒往往会絮凝或团聚。对于 PSS 颗粒，毒性与两种尺寸的纳米棒的表面积相关。
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