• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

I型胶原蛋白包被的金纳米棒与LED照射对人皮肤成纤维细胞伤口愈合的协同作用。

Synergistic effect of gold nanorods coated with type I collagen and LED irradiation on wound healing in human skin fibroblast cells.

作者信息

Poomrattanangoon Sasiprapa, Pissuwan Dakrong

机构信息

Materials Science and Engineering Program, Faculty of Science, Mahidol University Bangkok 10400 Thailand.

Nanobiotechnology and Nanobiomaterials Research Laboratory, School of Materials Science and Innovation, Faculty of Science, Mahidol University Bangkok 10400 Thailand

出版信息

Nanoscale Adv. 2025 May 22. doi: 10.1039/d4na01072h.

DOI:10.1039/d4na01072h
PMID:40417166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12096408/
Abstract

Delayed wound healing poses a significant risk to human health, especially when wounds are infected by pathogens. Therefore, the development of effective methods for accelerating wound healing is important. This study investigated the synergistic effect of gold nanorods (GNRs) coated with type I collagen (GNRs@C) combined with light-emitting diode (LED) irradiation on wound healing in scratched human skin fibroblast (HSF) cells. Scratched HSF cells were treated with 3 μg mL GNRs@C, followed by LED irradiation. This combined treatment significantly enhanced cell proliferation, increasing from the control cell base line (scratched HSF cells without any treatment) to 104.08 ± 2.96% and 107.82 ± 3.25% after 24 and 48 h of incubation, respectively. GNRs@C demonstrated superior cellular uptake compared to uncoated GNRs. Notably, complete closure of scratched HSF cells was observed in scratched HSF cells treated with GNRs@C with LED irradiation and then incubated for 40 h. Additionally, the treatment significantly reduced interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) levels, while upregulating key growth factors, including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). These findings demonstrate the wound healing potential of GNRs@C combined with LED irradiation.

摘要

伤口愈合延迟对人类健康构成重大风险，尤其是当伤口被病原体感染时。因此，开发有效的加速伤口愈合方法非常重要。本研究调查了涂有I型胶原蛋白的金纳米棒（GNRs@C）与发光二极管（LED）照射相结合对人皮肤成纤维细胞（HSF）划痕伤口愈合的协同作用。对HSF划痕细胞用3μg/mL GNRs@C处理，然后进行LED照射。这种联合处理显著增强了细胞增殖，与对照细胞基线（未进行任何处理的HSF划痕细胞）相比，孵育24小时和48小时后分别增加到104.08±2.96%和107.82±3.25%。与未涂层的GNRs相比，GNRs@C表现出更好的细胞摄取能力。值得注意的是，在用GNRs@C和LED照射处理的HSF划痕细胞中观察到划痕完全闭合，然后孵育40小时。此外，该处理显著降低了白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平，同时上调了包括血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）在内的关键生长因子。这些发现证明了GNRs@C与LED照射相结合促进伤口愈合的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/a1425ee39ce3/d4na01072h-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/c97fbe84a525/d4na01072h-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/5d678420e89b/d4na01072h-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/41688d9aa152/d4na01072h-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/1fec27e20e23/d4na01072h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/220cd5757a71/d4na01072h-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/9a7b5f15c608/d4na01072h-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/d637808ea78f/d4na01072h-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/e88711030cdf/d4na01072h-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/18cee7f480be/d4na01072h-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/f695ec1a8755/d4na01072h-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/a1425ee39ce3/d4na01072h-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/c97fbe84a525/d4na01072h-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/5d678420e89b/d4na01072h-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/41688d9aa152/d4na01072h-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/1fec27e20e23/d4na01072h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/220cd5757a71/d4na01072h-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/9a7b5f15c608/d4na01072h-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/d637808ea78f/d4na01072h-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/e88711030cdf/d4na01072h-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/18cee7f480be/d4na01072h-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/f695ec1a8755/d4na01072h-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/acdb/12151064/a1425ee39ce3/d4na01072h-f11.jpg

相似文献

1
Synergistic effect of gold nanorods coated with type I collagen and LED irradiation on wound healing in human skin fibroblast cells.I型胶原蛋白包被的金纳米棒与LED照射对人皮肤成纤维细胞伤口愈合的协同作用。
Nanoscale Adv. 2025 May 22. doi: 10.1039/d4na01072h.
2
Gold nanoparticles coated with collagen-I and their wound healing activity in human skin fibroblast cells.涂有I型胶原蛋白的金纳米颗粒及其在人皮肤成纤维细胞中的伤口愈合活性。
Heliyon. 2024 Jun 20;10(13):e33302. doi: 10.1016/j.heliyon.2024.e33302. eCollection 2024 Jul 15.
3
254 nm Ultraviolet C Light Emitting Diode (LED) Radiation Enhances Wound Healing in a Rat Model of Full-Thickness Skin Defects.254纳米紫外线C发光二极管（LED）辐射可促进大鼠全层皮肤缺损模型的伤口愈合。
Lasers Surg Med. 2025 May 20. doi: 10.1002/lsm.70030.
4
Exploring the Safety and Efficacy of Organic Light-Emitting Diode in Skin Rejuvenation and Wound Healing.探索有机发光二极管在皮肤年轻化和伤口愈合中的安全性和疗效。
Yonsei Med J. 2024 Feb;65(2):98-107. doi: 10.3349/ymj.2023.0125.
5
Light-emitting diode irradiation promotes donor site wound healing of the free gingival graft.发光二极管照射可促进游离龈瓣供区伤口愈合。
J Periodontol. 2015 May;86(5):674-81. doi: 10.1902/jop.2015.140580. Epub 2015 Jan 29.
6
Bioinspired Collagen Scaffold Loaded with bFGF-Overexpressing Human Mesenchymal Stromal Cells Accelerating Diabetic Skin Wound Healing via HIF-1 Signal Pathway Regulated Neovascularization.仿生胶原支架负载过表达 bFGF 的人骨髓间充质干细胞通过 HIF-1 信号通路调控新生血管化加速糖尿病皮肤创面愈合。
ACS Appl Mater Interfaces. 2024 Sep 4;16(35):45989-46004. doi: 10.1021/acsami.4c08174. Epub 2024 Aug 21.
7
Effects of 630 nm Red and 460 nm Blue Light Emitting Diode Irradiation on Healing of the Skin Wound in Japanese Big-ear White Rabbit.630纳米红光和460纳米蓝光发光二极管照射对日本大耳白兔皮肤伤口愈合的影响
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2017 Jun 20;39(3):301-306. doi: 10.3881/j.issn.1000-503X.2017.03.001.
8
[Effects of exosomes from human adipose-derived mesenchymal stem cells on inflammatory response of mouse RAW264.7 cells and wound healing of full-thickness skin defects in mice].人脂肪间充质干细胞来源外泌体对小鼠RAW264.7细胞炎症反应及小鼠全层皮肤缺损创面愈合的影响
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi. 2022 Mar 20;38(3):215-226. doi: 10.3760/cma.j.cn501120-20201116-00477.
9
[Mechanism of tannins from Galla chinensis cream in promoting skin wound healing in rats based on FAK/PI3K/Akt/mTOR signaling pathway].基于FAK/PI3K/Akt/mTOR信号通路探讨五倍子乳膏中鞣质促进大鼠皮肤创伤愈合的机制
Zhongguo Zhong Yao Za Zhi. 2025 Jan;50(2):480-497. doi: 10.19540/j.cnki.cjcmm.20240902.706.
10
Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression.从尼罗罗非鱼（Oreochromis niloticus L.）皮中提取的胶原蛋白通过上调 VEGF、bFGF 和 α-SMA 基因的表达促进大鼠模型的伤口愈合。
BMC Vet Res. 2020 Sep 24;16(1):352. doi: 10.1186/s12917-020-02566-2.

本文引用的文献

1
Red-light LED therapy promotes wound regeneration by upregulating COL1A1, COL2A1, VEGF and reducing IL-1β for anti-inflammation.红光发光二极管疗法通过上调COL1A1、COL2A1、血管内皮生长因子（VEGF）以及减少白细胞介素-1β（IL-1β）以抗炎，从而促进伤口再生。
Lasers Med Sci. 2025 Apr 3;40(1):171. doi: 10.1007/s10103-025-04432-9.
2
Collagen-Based Nanoparticles as Drug Delivery System in Wound Healing Applications.基于胶原蛋白的纳米粒子作为药物传递系统在创伤愈合中的应用。
Int J Nanomedicine. 2024 Nov 6;19:11321-11341. doi: 10.2147/IJN.S485588. eCollection 2024.
3
Photochemical Synthesis of Sericin-Coated Gold Nanorods and Their Antibacterial Activity under Low-Level Near-Infrared Light.
丝胶蛋白包覆的金纳米棒的光化学合成及其在低强度近红外光下的抗菌活性。
Langmuir. 2024 Oct 15;40(41):21795-21803. doi: 10.1021/acs.langmuir.4c02984. Epub 2024 Oct 4.
4
The influence of Type I and III collagen on the proliferation, migration and differentiation of myoblasts.Ⅰ型和Ⅲ型胶原对成肌细胞增殖、迁移和分化的影响。
Tissue Cell. 2024 Oct;90:102506. doi: 10.1016/j.tice.2024.102506. Epub 2024 Jul 30.
5
Gold nanoparticles coated with collagen-I and their wound healing activity in human skin fibroblast cells.涂有I型胶原蛋白的金纳米颗粒及其在人皮肤成纤维细胞中的伤口愈合活性。
Heliyon. 2024 Jun 20;10(13):e33302. doi: 10.1016/j.heliyon.2024.e33302. eCollection 2024 Jul 15.
6
Collagen peptides affect collagen synthesis and the expression of collagen, elastin, and versican genes in cultured human dermal fibroblasts.胶原蛋白肽影响培养的人皮肤成纤维细胞中胶原蛋白的合成以及胶原蛋白、弹性蛋白和多功能蛋白聚糖基因的表达。
Front Med (Lausanne). 2024 May 1;11:1397517. doi: 10.3389/fmed.2024.1397517. eCollection 2024.
7
Effect of a portable light emitting diode device on wound healing in a rat model.便携式发光二极管装置对大鼠模型伤口愈合的影响。
Int Wound J. 2024 Feb;21(2):e14335. doi: 10.1111/iwj.14335. Epub 2023 Oct 11.
8
Effect of red light on epidermal proliferation and mitochondrial activity.红光对表皮增殖和线粒体活性的影响。
Skin Res Technol. 2023 Sep;29(9):e13447. doi: 10.1111/srt.13447.
9
Isolation and Characterization of Collagen and Collagen Peptides with Hyaluronidase Inhibition Activity Derived from the Skin of Marlin ().从马林鱼（）皮中分离得到具有透明质酸酶抑制活性的胶原蛋白和胶原肽的分离与表征。
Molecules. 2023 Jan 16;28(2):889. doi: 10.3390/molecules28020889.
10
Overexpression of VEGF in dermal fibroblast cells accelerates the angiogenesis and wound healing function: in vitro and in vivo studies.真皮成纤维细胞中 VEGF 的过表达可加速血管生成和伤口愈合功能：体外和体内研究。
Sci Rep. 2022 Nov 2;12(1):18529. doi: 10.1038/s41598-022-23304-8.