Dijkhuizen L, Groen L, Harder W, Konings W N
Arch Microbiol. 1977 Nov 18;115(2):223-7. doi: 10.1007/BF00406378.
Membrane vesicles isolated from oxalate-grown cells of Pseudomonas oxalaticus accumulated oxalate by an inducible transport system in unmodified form against a concentration gradient. This accumulation was dependent on the presence of a suitable electron donor system such as ascorbate-phenazine-methosulphate. In the presence of this energy source, steady state levels of accumulation of oxalate were 10--20-fold higher than in its absence. The oxalate transport system involved showed a high affinity for oxalate (Km = 11 micron) and was highly specific. Oxalate transport was not affected by the presence of other dicarboxylic acids, such as malate, succinate and fumarate and only partly inhibited by acetate. The energy requirement for oxalate transport is discussed and it is concluded that this requirement is most likely equivalent to 1 mole of ATP per mole of oxalate.
从草酸假单胞菌的草酸生长细胞中分离出的膜泡，通过一种可诱导的转运系统以未修饰的形式逆浓度梯度积累草酸。这种积累依赖于合适的电子供体系统的存在，如抗坏血酸 - 吩嗪 - 甲硫酸盐。在这种能量来源存在的情况下，草酸积累的稳态水平比不存在时高10 - 20倍。所涉及的草酸转运系统对草酸具有高亲和力（Km = 11微米）且具有高度特异性。草酸转运不受其他二羧酸如苹果酸、琥珀酸和富马酸的存在的影响，仅部分受乙酸抑制。讨论了草酸转运的能量需求，并得出结论，这种需求最可能相当于每摩尔草酸1摩尔ATP。
