[大黄根茎的毛细管电泳-二极管阵列检测指纹图谱]
[MEKC-DAD fingerprint of Radix et Rhizoma Rhei].
作者信息
Liu Xunhong, Li Junsong, Zhang Yuechan, Cai Baochang, Di Yin
机构信息
Pharmacology College, Nanjing University of Chinese Medicine, Nangjing 210046, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Dec;34(23):3034-8.
OBJECTIVE
To establish the analytical method for the fingerprint of Radix et Rhizoma Rhei by MEKC-DAD and compare the fingerprints of Radix et Rhizoma Rhei and its processed products.
METHOD
Based on the mode of micellar electrokinetic chromatography, 25 mmol x L(-1) borax -25 mmol x L(-1) SDS-10% acetonitrile was selected for the running buffer (pH 9.2). The separation voltage was 12 kV and the detection wavelength was set at 254 nm. Rhein was used as a reference standard, the chromatographic fingerprint was determined via the data analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples.
RESULT
MEKC-DAD fingerprints with 11 common peaks of 10 batches of Radix et Rhizoma Rhei from the place of the genuine were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Radix et Rhizoma Rhei and its processed products, there were obvious differences in the relative areas of common peaks.
CONCLUSION
The method is reliable, accurate and can be used for quality control of Radix et Rhizoma Rhei.
目的
建立大黄药材的胶束电动毛细管色谱-二极管阵列检测法（MEKC-DAD）指纹图谱分析方法，并比较大黄药材及其炮制品的指纹图谱。
方法
基于胶束电动色谱模式，选用25 mmol·L⁻¹硼砂-25 mmol·L⁻¹十二烷基硫酸钠-10%乙腈作为运行缓冲液（pH 9.2）。分离电压为12 kV，检测波长设定为254 nm。以大黄酸为对照品，通过模糊聚类和指纹图谱相似度评价软件对数据进行分析，确定色谱指纹图谱，比较样品的相似度。
结果
初步建立了10批道地药材大黄的具有11个共有峰的MEKC-DAD指纹图谱。发现少数样品与其他样品存在差异。对于大黄药材及其炮制品的指纹图谱，共有峰的相对面积存在明显差异。
结论
该方法可靠、准确，可用于大黄的质量控制。
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