[Study on HPCE fingerprint of Eucommiae Cortex].

OBJECTIVE

To establish the analytical method for the fingerprint of Eucommiae Cortex by HPCE and compare the fingerprints of Eucommiae Cortex and its processed products.

METHODS

Based on the mode of high performance capillary electrophoresis, 60 mmol/L sodium borate-20 mmol/L sodium dihydrogen phosphate-10% methanol (pH 10.0) was used as buffer solution. The separation voltage was 20 kV and the detection wavelength was set at 210 nm. Pinoresinol diglucoside was used as a reference standard, the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation softwarewas used to compare the similarity of samples.

RESULTS

HPCE fingerprints with 10 common peaks of Eucommiae Cortex were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Eucommiae Cortex and its processed products, there were obvious differences in the relative areas of common peaks.

CONCLUSION

The method is reliable, accurate and can be used for quality control of Eucommiae Cortex.