[地黄高效毛细管电泳指纹图谱研究]
[Study on the HPCE fingerprint of Rehmannia glutinosa].
作者信息
Xu Hu, Han Le, Liu Xun-Hong, Fu Xing-Sheng, Li Jun-Song, Cai Bao-Chang, Shen Ze-Zhong
机构信息
Nanjing University of Chinese Medicine, Nanjing 210046, China.
出版信息
Zhong Yao Cai. 2012 Apr;35(4):548-52.
OBJECTIVE
To establish the analytical method for the fingerprint of Rehmannia glutinosa by HPCE and compare the fingerprints of Rehmannia glutinosa and its processed products.
METHODS
Based on the mode of high performance capillary electrophoresis, 60 mmol/L sodium borate was used as buffer solution (5% MeOH, pH 9.5). The separation voltage was 20 kV and the detection wavelength was set at 210 nm. Catalpol was used as a reference standard, the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software was used to compare the similarity of samples.
RESULTS
HPCE fingerprints with 7 common peaks of Rehmannia glutinosa were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Rehmannia glutinosa and its processed products, there were obvious differences in the relative areas of common peaks.
CONCLUSION
The method is reliable, accurate and can be used for quality control of Rehmannia glutinosa.
目的
建立高效毛细管电泳法测定地黄指纹图谱的分析方法，并比较地黄及其炮制品的指纹图谱。
方法
基于高效毛细管电泳模式，以60 mmol/L硼酸钠为缓冲溶液（含5%甲醇，pH 9.5）。分离电压为20 kV，检测波长设定为210 nm。以梓醇为对照品，测定色谱指纹图谱。采用模糊聚类分析数据，并用指纹图谱相似度评价软件比较样品相似度。
结果
初步建立了地黄具有7个共有峰的高效毛细管电泳指纹图谱。发现少数样品与其他样品不同。关于地黄及其炮制品的指纹图谱，共有峰的相对面积存在明显差异。
结论
该方法可靠、准确，可用于地黄的质量控制。
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