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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Bertrand F, Hermelin B, Paul A, Picard J

Laboratoire de Biochimie, I.N.S.E.R.M.-U. 181, Faculté de Médecine Saint-Antoine, Paris, France.

Int J Biochem. 1991;23(1):51-7. doi: 10.1016/0020-711x(91)90008-b.

Labeled glycoconjugates released by trypsin from cell surfaces of control and cystic fibrosis (CF) skin fibroblasts were purified and fractionated by column chromatography on Sephadex G-50 and Concanavalin A Sepharose. Based on chemical analysis and specific enzymatic digestions: (1) Glycoconjugates were characterized as O-linked glycopeptides consisting predominantly of glycosaminoglycan type and N-linked glycopeptides with glycans of complex type. Their relative proportions were similar between the two groups. (2) The N-linked glycopeptides exhibited an increased molar ratio of fucose to galactose in CF fibroblasts. (3) When pericellular glycoconjugates were metabolically labeled with [14C]glucosamine and [3H]fucose, incorporation and degradation kinetics were similar between the two groups.

用胰蛋白酶从对照和囊性纤维化（CF）皮肤成纤维细胞表面释放的标记糖缀合物，经葡聚糖G-50和伴刀豆球蛋白A琼脂糖柱色谱法纯化和分级分离。基于化学分析和特异性酶消化：（1）糖缀合物被鉴定为主要由糖胺聚糖类型的O-连接糖肽和具有复杂型聚糖的N-连接糖肽组成。两组之间它们的相对比例相似。（2）CF成纤维细胞中N-连接糖肽的岩藻糖与半乳糖的摩尔比增加。（3）当用[14C]葡萄糖胺和[3H]岩藻糖对细胞周围糖缀合物进行代谢标记时，两组之间的掺入和降解动力学相似。

Bertrand F, Hermelin B, Paul A, Picard J

Laboratoire de Biochimie, I.N.S.E.R.M.-U. 181, Faculté de Médecine Saint-Antoine, Paris, France.

Int J Biochem. 1991;23(1):51-7. doi: 10.1016/0020-711x(91)90008-b.

Labeled glycoconjugates released by trypsin from cell surfaces of control and cystic fibrosis (CF) skin fibroblasts were purified and fractionated by column chromatography on Sephadex G-50 and Concanavalin A Sepharose. Based on chemical analysis and specific enzymatic digestions: (1) Glycoconjugates were characterized as O-linked glycopeptides consisting predominantly of glycosaminoglycan type and N-linked glycopeptides with glycans of complex type. Their relative proportions were similar between the two groups. (2) The N-linked glycopeptides exhibited an increased molar ratio of fucose to galactose in CF fibroblasts. (3) When pericellular glycoconjugates were metabolically labeled with [14C]glucosamine and [3H]fucose, incorporation and degradation kinetics were similar between the two groups.

用胰蛋白酶从对照和囊性纤维化（CF）皮肤成纤维细胞表面释放的标记糖缀合物，经葡聚糖G-50和伴刀豆球蛋白A琼脂糖柱色谱法纯化和分级分离。基于化学分析和特异性酶消化：（1）糖缀合物被鉴定为主要由糖胺聚糖类型的O-连接糖肽和具有复杂型聚糖的N-连接糖肽组成。两组之间它们的相对比例相似。（2）CF成纤维细胞中N-连接糖肽的岩藻糖与半乳糖的摩尔比增加。（3）当用[14C]葡萄糖胺和[3H]岩藻糖对细胞周围糖缀合物进行代谢标记时，两组之间的掺入和降解动力学相似。