Novak R A, Abell C W
Tex Rep Biol Med. 1976;34(1):199-207.
The capacity of skin fibroblasts from cystic fibrosis (CF) and non-CF individuals to incorporate exogenous L-fucose into various metabolic pools was compared. Incorporation into intracellular acid-soluble pools and extracellular glycopeptides was measured by a dual-label co-chromatographic technique, whereas total macromolecular incorporation was measured by a single-label procedure. Although CF and normal fibroblasts incorporate exogenous fucose into intracellular acid-soluble and high molecular weight compounds in a similar manner, CF cells appear to be characterized by an increased incorporation of label into low molecular weight extracellular glycopeptides.
比较了囊性纤维化（CF）患者和非CF个体的皮肤成纤维细胞将外源性L-岩藻糖掺入各种代谢池的能力。通过双标记共色谱技术测量其掺入细胞内酸溶性池和细胞外糖肽的情况，而通过单标记程序测量总的大分子掺入情况。尽管CF成纤维细胞和正常成纤维细胞以相似的方式将外源性岩藻糖掺入细胞内酸溶性和高分子量化合物中，但CF细胞的特征似乎是标记物更多地掺入低分子量细胞外糖肽中。
