Department of Cardiology, Institute of Cardiovascular Diseases, First Affiliated Hospital, 22 Shuangyong Road, Nanning 530021, Guangxi, People's Republic of China.
Lipids Health Dis. 2010 Mar 11;9:28. doi: 10.1186/1476-511X-9-28.
BACKGROUND: The association between -250G>A polymorphism in the promoter region of the hepatic lipase gene (LIPC) and plasma high-density lipoprotein cholesterol (HDL-C) concentration is contradictory in diverse ethnics. Bai Ku Yao is an isolated subgroup of the Yao minority in China. This study was designed to detect the association of LIPC -250G>A (rs2070895) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. METHODS: A total of 778 subjects of Bai Ku Yao and 648 participants of Han Chinese aged 15-80 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the LIPC -250G>A was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. RESULTS: The levels of serum total cholesterol (TC), HDL-C, low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) AI were lower in Bai Ku Yao than in Han (P < 0.01 for all). The frequencies of GG, GA and AA genotypes were 50.0%, 43.3% and 6.7% in Bai Ku Yao, and 35.7%, 50.6% and 13.7% in Han (P < 0.01); respectively. The frequencies of G and A alleles were 71.7% and 28.3% in Bai Ku Yao, and 61.0% and 39.0% in Han (P < 0.01). The levels of HDL-C and the ratio of ApoAI to ApoB in Bai Ku Yao were lower in GG genotype than in GA or AA genotype (P < 0.05-0.01). The levels of TC, HDL-C, LDL-C and ApoB in Han were lower in GG genotype than in GA or AA genotype (P < 0.05-0.01). The levels of HDL-C and the ratio of ApoAI to ApoB in Bai Ku Yao, and the levels of HDL-C, LDL-C and ApoB in Han were correlated with genotype and/or allele (P < 0.05 for all). Serum lipid parameters were also correlated with age, sex, alcohol consumption, cigarette smoking, blood pressure, body weight, and body mass index in both ethnic groups. CONCLUSIONS: The differences in the serum lipid profiles between the two ethnic groups might partly result from different genotypic frequency of LIPC -250G>A or different LIPC-enviromental interactions.
背景：肝脂肪酶基因（LIPC）启动子区域-250G>A 多态性与血浆高密度脂蛋白胆固醇（HDL-C）浓度之间的关联在不同种族中存在争议。白裤瑶是中国瑶族的一个孤立的分支群体。本研究旨在检测 LIPC-250G>A（rs2070895）多态性与几个环境因素与广西白裤瑶和汉族人群血清脂质水平的关系。 方法：从我们之前的分层随机聚类样本中随机抽取 778 名白裤瑶和 648 名汉族 15-80 岁的受试者。采用聚合酶链反应和限制性片段长度多态性结合凝胶电泳检测 LIPC-250G>A 的基因型，然后通过直接测序进行确认。 结果：白裤瑶人群的血清总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）和载脂蛋白（Apo）AI 水平均低于汉族（均 P<0.01）。白裤瑶人群的 GG、GA 和 AA 基因型频率分别为 50.0%、43.3%和 6.7%，汉族分别为 35.7%、50.6%和 13.7%（均 P<0.01）；白裤瑶人群的 G 和 A 等位基因频率分别为 71.7%和 28.3%，汉族分别为 61.0%和 39.0%（均 P<0.01）。白裤瑶人群 GG 基因型的 HDL-C 水平和载脂蛋白 AI 与载脂蛋白 B 的比值均低于 GA 或 AA 基因型（P<0.05-0.01）。汉族人群 GG 基因型的 TC、HDL-C、LDL-C 和 ApoB 水平均低于 GA 或 AA 基因型（P<0.05-0.01）。白裤瑶人群的 HDL-C 水平和载脂蛋白 AI 与载脂蛋白 B 的比值，以及汉族人群的 HDL-C、LDL-C 和 ApoB 水平与基因型和/或等位基因相关（均 P<0.05）。两组人群的血清脂质参数也与年龄、性别、饮酒、吸烟、血压、体重和体重指数相关。 结论：两组人群的血清血脂谱差异可能部分归因于 LIPC-250G>A 的不同基因型频率或不同的 LIPC-环境相互作用。
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