Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
Science. 2010 Mar 26;327(5973):1644-8. doi: 10.1126/science.1184008. Epub 2010 Mar 11.
Extracellular matrices in diverse biological systems are cross-linked by dityrosine covalent bonds catalyzed by the peroxidase/oxidase system. We show that a peroxidase, secreted by the Anopheles gambiae midgut, and dual oxidase form a dityrosine network that decreases gut permeability to immune elicitors. This network protects the microbiota by preventing activation of epithelial immunity. It also provides a suitable environment for malaria parasites to develop within the midgut lumen without inducing nitric oxide synthase expression. Disruption of this barrier results in strong and effective pathogen-specific immune responses.
不同生物系统中的细胞外基质通过过氧化物酶/氧化酶系统催化的二酪氨酸共价键交联。我们表明,冈比亚按蚊中肠分泌的一种过氧化物酶和双氧化酶形成了一个二酪氨酸网络,降低了肠道对免疫激发剂的通透性。该网络通过防止上皮免疫的激活来保护微生物区系。它还为疟原虫在中肠腔中发育提供了一个合适的环境,而不会诱导一氧化氮合酶的表达。破坏这种屏障会导致强烈而有效的针对病原体的免疫反应。