Kum K, Khanna R, Vinayak V K
Department of Experimental Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh.
Indian J Med Res. 1991 Jan;93:40-6.
Two surface associated antigens (GLSA-82 and GLSA-56) of axenically grown G. lamblia trophozoites (PI strain) were affinity purified from its sonic extract. Both GLSA-82 and GLSA-56 were heat labile, sensitive to treatment with pronase, trypsin and were also sodium metaperiodate modifiable as assessed by micro ELISA. Lectin binding studies revealed that GLSA-82 specifically bound concanavalin A and pokeweed mitogen, and had alpha-methyl mannoside and n-acetyl-B-d-glucosamine sugar moieties. However, GLSA-56 selectively bound Ricinus communis agglutinin and phytohaemagglutinin, and had B-d-galactose and n-acetyl-B-d-galastosamine as sugar moieties. Human sera obtained during acute G. lamblia infection recognised GLSA-82 and GLSA-56 antigens. However, the antibody levels to GLSA-82 were significantly lower (P less than 0.05) during active giardiasis infection. Such surface associated antigens may be target of antiparasitic immune responses and thus, may modulate disease processes.
从无共生培养的蓝氏贾第鞭毛虫滋养体（PI株）的超声提取物中亲和纯化出两种表面相关抗原（GLSA - 82和GLSA - 56）。通过微量酶联免疫吸附测定评估，GLSA - 82和GLSA - 56均对热不稳定，对链霉蛋白酶、胰蛋白酶处理敏感，并且也可被高碘酸钠修饰。凝集素结合研究表明，GLSA - 82特异性结合伴刀豆球蛋白A和商陆有丝分裂原，并且具有α - 甲基甘露糖苷和N - 乙酰 - β - D - 葡糖胺糖部分。然而，GLSA - 56选择性结合蓖麻凝集素和植物血凝素，并且具有β - D - 半乳糖和N - 乙酰 - β - D - 半乳糖胺作为糖部分。在急性蓝氏贾第鞭毛虫感染期间获得的人血清可识别GLSA - 82和GLSA - 56抗原。然而，在活动性贾第虫病感染期间，针对GLSA - 82的抗体水平显著较低（P小于0.05）。此类表面相关抗原可能是抗寄生虫免疫反应的靶点，因此可能调节疾病进程。
