Yang Junxing, Hua Qunyi, Chen Huanchun, Lv Jianqiang, Chen Bing, Ruan Zhouxi
No. 2049, Heping Road, Shenzhen, Guangdong, P. R. China 518001.
J Vet Diagn Invest. 2010 Mar;22(2):242-4. doi: 10.1177/104063871002200212.
A latex agglutination test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa = 0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.
