Chand Karam, Biswas Sanchay K, Pandey Awadh B, Saxena Arpit, Tewari Neha, Mondal Bimalendu
Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital, 263 138, Uttarakhand, India.
Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital, 263 138, Uttarakhand, India.
Biologicals. 2017 Mar;46:168-171. doi: 10.1016/j.biologicals.2017.01.002. Epub 2017 Jan 19.
Bluetongue virus (BTV) is transmitted by biting midges, which infects domestic and wild ruminants. In present study, a competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of serogroup-specific antibodies against VP7 protein of BTV has been developed. The assay measures the competition between a group specific antibody against core protein of BTV and a test serum to an optimized concentration of BTV recombinant-VP7 (r-VP7) antigen. Serum samples (n = 895) collected from small and large ruminants were used to optimize the C-ELISA. Percent inhibition (PI) values were used for estimation of the cut-off value for the C-ELISA. On receiver operator characteristic (ROC) analysis, different cut-off values along with their diagnostic sensitivity (DSn) and diagnostic specificity (DSp) were obtained. Among these, >50% PI value was accepted as cut-off at which DSn and Dsp was achieved as 97.6% and 98.0% respectively, at >95% confidence interval. Results show the present C-ELISA assay described to be sensitive, specific and reliable and could be adopted for serological investigation of small and large ruminants.
蓝舌病病毒（BTV）通过叮咬蠓传播，可感染家养和野生反刍动物。在本研究中，已开发出一种用于检测针对BTV VP7蛋白的血清群特异性抗体的竞争酶联免疫吸附测定（C-ELISA）。该测定法测量针对BTV核心蛋白的群特异性抗体与测试血清对优化浓度的BTV重组VP7（r-VP7）抗原的竞争情况。从小反刍动物和大反刍动物收集的血清样本（n = 895）用于优化C-ELISA。抑制百分比（PI）值用于估计C-ELISA的临界值。通过受试者工作特征（ROC）分析，获得了不同的临界值及其诊断敏感性（DSn）和诊断特异性（DSp）。其中，PI值>50%被接受为临界值，在此临界值下，在>95%置信区间时，DSn和Dsp分别达到97.6%和98.0%。结果表明，所描述的当前C-ELISA测定法灵敏、特异且可靠，可用于小反刍动物和大反刍动物的血清学调查。
