Department of Public Health Sciences, Sapienza University, Rome, Italy.
J Physiol Pharmacol. 2009 Dec;60 Suppl 6:19-26.
The protective effect of selected vaginal Lactobacillus strains (L. brevis CD2, L. salivarius FV2, L. plantarum FV9) towards herpes simplex virus type 2 (HSV-2) infection in vitro has been analyzed. Living bacterial cells affect different steps of virus multiplication. The effect on the early phases of virus infection appeared related to the bacterial adhesive potential to the cell membrane while all the strains strongly reduced intracellular events of virus multiplication. The anti HSV-2 activity was not mediated by a virucidal effect. Instead it was exerted through bacterial soluble factors able to down regulate the production of infective virions. In fact HSV-2 yield was significantly reduced in infected cells fed with cell-free supernatants of lactobacilli grown in cell culture medium. Purified lactic acid and H(2)O(2), Lactobacillus metabolites with known antimicrobial activity, produced a dose-dependent virucidal effect. Lactic acid successfully interfered with viral intracellular antigen synthesis and both the virucidal activity and the inhibition of replication were correlated to acidic pH values. L. brevis CD2, the most active strain, does not produce H(2)O(2) and neutralized lactic acid had no effect, thus indicating that factors other than H(2)O(2) and lactic acid could be responsible for the antiviral effect.
已分析选定阴道乳杆菌菌株（L. brevis CD2、L. salivarius FV2、L. plantarum FV9）对单纯疱疹病毒 2 型（HSV-2）体外感染的保护作用。活细菌细胞影响病毒复制的不同步骤。对病毒感染早期阶段的影响似乎与细菌对细胞膜的粘附潜力有关，而所有菌株均强烈降低了病毒复制的细胞内事件。抗 HSV-2 活性不是通过病毒杀伤作用介导的。相反，它是通过能够下调感染性病毒颗粒产生的细菌可溶性因子发挥作用的。事实上，在用细胞培养物中生长的乳杆菌的无细胞上清液喂养感染细胞时，HSV-2 的产量明显降低。具有已知抗菌活性的乳酸和 H2O2 等乳酸杆菌代谢物产生了剂量依赖性的病毒杀伤作用。乳酸成功地干扰了病毒的细胞内抗原合成，病毒杀伤活性和复制抑制与酸性 pH 值相关。最活跃的菌株 L. brevis CD2 不产生 H2O2，中和的乳酸没有效果，因此表明除 H2O2 和乳酸之外的其他因素可能是抗病毒作用的原因。
