Kassaa Imad Al, Hober Didier, Hamze Monzer, Caloone Delphine, Dewilde Anny, Chihib Nour-Eddine, Drider Djamel
Université de Lille 1 Sciences et Technologies, Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies, Institut Charles Viollette-Université Lille 1, Bâtiment Polytech-Lille, Bâtiment Polytech-Lille (Bureau C315), Cité Scientifique, Université de Lille, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France.
Arch Microbiol. 2015 Jun;197(5):657-64. doi: 10.1007/s00203-015-1101-8. Epub 2015 Mar 10.
This study aimed at demonstrating the antiviral activity of Lactobacillus gasseri CMUL57 (L. gasseri CMUL57), L. acidophilus CMUL67 and L. plantarum CMUL140 against herpes simplex type 2 (HSV-2) and Coxsackievirus B4E2 (CVB4E2), which are enveloped and naked viruses, respectively. These lactobacilli were non-cytotoxic and were able to reduce the cytopathic effect induced by HSV-2 in Vero cell monolayers. However, lactobacilli were not active against CVB4E2. Tested lactobacilli displayed anti-HSV-2 activity when they were co-incubated with the virus prior to inoculating the mixture to Vero cell monolayers. The detection of HSV-2 DNA by PCR in pellets of bacteria/virus mixtures let us to hypothesize that anti-HSV-2 activity of lactobacilli resulted from the viruses' entrapment. This study showed the capabilities of vaginal lactobacilli to inhibit enveloped viruses such as HSV-2.
本研究旨在证明加氏乳杆菌CMUL57（加氏乳杆菌CMUL57）、嗜酸乳杆菌CMUL67和植物乳杆菌CMUL140分别对包膜病毒单纯疱疹病毒2型（HSV - 2）和无包膜病毒柯萨奇病毒B4E2（CVB4E2）的抗病毒活性。这些乳杆菌无细胞毒性，且能够降低HSV - 2在Vero细胞单层中诱导的细胞病变效应。然而，乳杆菌对CVB4E2无活性。当将受试乳杆菌与病毒共同孵育后再接种至Vero细胞单层时，它们表现出抗HSV - 2活性。通过PCR检测细菌/病毒混合物沉淀中的HSV - 2 DNA，使我们推测乳杆菌的抗HSV - 2活性源于病毒的截留。本研究表明阴道乳杆菌具有抑制包膜病毒如HSV - 2的能力。
