Department of Embryology and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium.
In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):300-8. doi: 10.1007/s11626-010-9284-4. Epub 2010 Mar 12.
In this report, we present the derivation and characterization of 15 hESC lines established at the Vrije Universiteit Brussel, Belgium in collaboration with the Universitair Ziekenhuis Brussel, Belgium, using surplus in vitro fertilization embryos and embryos carrying monogenic disorders donated for research. Four lines were derived from blastocyst-stage embryos presumed to be genetically normal, and 11 hESC lines were obtained from embryos shown to carry genetic mutations by preimplantation genetic diagnosis. All the lines express markers of pluripotency as determined by immunocytochemistry and RT-PCR, and formed teratomas when injected into SCID mice. All VUB hESC lines, except for VUB17, are reported in the European hESC registry and are available upon request after signing a Material Transfer Agreement from the VUB (contact person: Prof. Dr. Karen Sermon; Karen.Sermon@uzbrussel.be).
在本报告中，我们介绍了比利时布鲁塞尔自由大学与比利时布鲁塞尔大学附属医院合作，利用体外受精胚胎和捐赠用于研究的携带单基因疾病的胚胎，建立的 15 株 hESC 系的衍生和特征。其中 4 株系来源于囊胚期胚胎，假定为遗传正常，11 株系来源于通过胚胎植入前遗传学诊断显示携带基因突变的胚胎。所有系均通过免疫细胞化学和 RT-PCR 鉴定表达多能性标志物，并在注射到 SCID 小鼠中形成畸胎瘤。除 VUB17 外，所有 VUB hESC 系均已在欧洲 hESC 注册中心注册，并可在签署 VUB 的材料转让协议后（联系人：Karen Sermon 教授博士；Karen.Sermon@uzbrussel.be）提出请求。
