Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
J Ind Microbiol Biotechnol. 2010 Jun;37(6):587-94. doi: 10.1007/s10295-010-0705-x. Epub 2010 Mar 12.
Pullulan is a linear homopolysaccharide that is composed of glucose units and often described as alpha-1, 6-linked maltotriose. In this study, response surface methodology using Box-Behnken design was employed to study the effects of sucrose and nitrogen concentrations on pullulan production. A total of 15 experimental runs were carried out in a plastic composite support biofilm reactor. Three-dimensional response surface was generated to evaluate the effects of the factors and to obtain the optimum condition of each factor for maximum pullulan production. After 7-day fermentation with optimum condition, the pullulan production reached 60.7 g/l, which was 1.8 times higher than the result from initial medium, and was the highest yield reported to date. The quality analysis demonstrated that the purity of produced pullulan was 95.2%, and its viscosity was 2.5 centipoise (cP), which is higher than the commercial pullulan and related to its molecular weight. Fourier transform infrared spectroscopy (FTIR) also suggested that the produced exopolysaccharide was pullulan.
普鲁兰是一种由葡萄糖单元组成的线性同多糖，通常描述为α-1,6-连接的麦芽三糖。在这项研究中，使用 Box-Behnken 设计的响应面法被用于研究蔗糖和氮浓度对普鲁兰生产的影响。在塑料复合支撑生物膜反应器中进行了总共 15 次实验运行。生成了三维响应面，以评估因素的影响，并获得每个因素的最佳条件，以获得最大普鲁兰产量。在最佳条件下发酵 7 天后，普鲁兰的产量达到 60.7 g/L，比初始培养基的结果高 1.8 倍，是迄今为止报道的最高产量。质量分析表明，所生产的普鲁兰的纯度为 95.2%，其粘度为 2.5 厘泊（cP），高于商业普鲁兰，与其分子量有关。傅里叶变换红外光谱（FTIR）也表明，所产生的胞外多糖是普鲁兰。
