School of Chemical and Biotechnology, SASTRA University, Thanjavur - 613401, Tamilnadu, India.
Carbohydr Polym. 2013 Jan 30;92(1):697-703. doi: 10.1016/j.carbpol.2012.09.062. Epub 2012 Oct 2.
Production of a commercially important biodegradable polymer, pullulan, by Aureobasidium pullulans from four agricultural wastes namely wheat bran, rice bran, coconut kernel and palm kernel was evaluated in solid state fermentation. Under the experimental conditions, palm kernel resulted in highest concentration of pullulan (16 g/L) among the four solid substrates. Optimum initial pH and moisture content for pullulan production were found out to be 6.5 and 50% respectively. 18.43 g/L of pullulan was produced from Asian palm kernel with initial pH 6.5 after 7 days of fermentation and yeast like morphology was predominant under this condition. Among different nitrogen sources tried in this study, yeast extract was found to the best. The pullulan produced from palm kernel was characterized by FTIR and (1)H NMR. The results were matching with that of commercial pullulan. Thus, Asian palm kernel appears to be an attractive low cost carbon source for the production of pullulan.
以四种农业废弃物（麦麸、米糠、椰子仁、棕榈仁）为原料，通过出芽短梗霉固态发酵生产具有商业重要性的可生物降解聚合物——普鲁兰多糖。在实验条件下，棕榈仁在四种固体基质中产生了最高浓度的普鲁兰多糖（16 g/L）。最佳初始 pH 值和产普鲁兰多糖的最佳水分含量分别为 6.5 和 50%。在 7 天的发酵后，用初始 pH 值为 6.5 的亚洲棕榈仁生产了 18.43 g/L 的普鲁兰多糖，在此条件下，酵母样形态占主导地位。在本研究中尝试的不同氮源中，酵母提取物效果最佳。从棕榈仁中提取的普鲁兰多糖的特性通过傅里叶变换红外光谱（FTIR）和（1）H 核磁共振（NMR）进行了表征。结果与商业普鲁兰多糖相匹配。因此，亚洲棕榈仁似乎是生产普鲁兰多糖的一种有吸引力的低成本碳源。
