Department of Orthodontics, Academic Center for Dentistry Amsterdam, University of Amsterdam and VU University, Amsterdam, The Netherlands.
Calcif Tissue Int. 2010 Apr;86(4):307-12. doi: 10.1007/s00223-010-9339-2. Epub 2010 Mar 12.
Collagen is an important constituent of bone, and it has been suggested that changes in collagen and mineral properties of bone are interrelated during growth. The aim of this study was to quantify age-related changes in collagen properties and the degree of mineralization of bone (DMB). The DMB in cancellous and cortical bone samples from the mandibular condyle of 35 female pigs aged 0-100 weeks was determined using micro-computed tomography. Subsequently, the amount of collagen and the number of pentosidine (Pen), hydroxylysylpyridinoline (HP), and lysylpyridinoline (LP) cross-links were quantified by means of high-performance liquid chromatography. The amount of collagen increased with age in cancellous bone but remained unchanged in cortical bone. The number of Pen and LP cross-links decreased in both bone types. In contrast, the number of HP cross-links decreased only in cancellous bone. The sum of the number of HP and LP cross-links decreased with age in cancellous bone only. The DMB increased in cancellous and cortical bone. It was concluded that the largest changes in the number of mature collagen cross-links and the mineralization in porcine cancellous and cortical bone take place before the age of 40 weeks. The low number of mature cross-links after this age suggests that the bone turnover rate continues to be high and thereby prevents the development of mature cross-links.
胶原蛋白是骨骼的重要组成部分，有人认为，在生长过程中，胶原蛋白和骨骼矿物质性质的变化是相互关联的。本研究的目的是定量研究胶原蛋白特性和骨矿化程度（DMB）随年龄的变化。使用微计算机断层扫描技术，测定了来自 35 只 0-100 周龄雌性猪下颌髁突的松质骨和皮质骨样本中的 DMB。随后，通过高效液相色谱法定量测定了胶原蛋白的含量以及戊糖素（Pen）、羟赖氨酸吡啶啉（HP）和赖氨酰吡啶啉（LP）交联的数量。松质骨中的胶原蛋白含量随年龄的增加而增加，但皮质骨中的胶原蛋白含量保持不变。两种骨类型中 Pen 和 LP 交联的数量均减少。相比之下，HP 交联的数量仅在松质骨中减少。只有在松质骨中，HP 和 LP 交联的数量之和随年龄的增加而减少。松质骨和皮质骨的 DMB 均增加。结论是，在 40 周龄之前，猪的松质骨和皮质骨中成熟胶原蛋白交联数量和矿化程度的最大变化发生。在此年龄之后，成熟交联数量较少表明骨周转率仍然很高，从而防止了成熟交联的形成。
