Eyre D R, Dickson I R, Van Ness K
Department of Orthopaedics, University of Washington, Seattle 98195.
Biochem J. 1988 Jun 1;252(2):495-500. doi: 10.1042/bj2520495.
The concentration in collagen of hydroxypyridinium cross-linking amino acids was measured in samples of bone and cartilage from human subjects aged from 1 month to 80 years. Cortical and cancellous bone samples were dissected and analysed separately. In both bone and cartilage, the content of this mature form of cross-link reached a maximum by 10-15 years of age (the amount in cartilage being 5-10 times that in bone), then stayed essentially in the same range throughout adult life. In bone the ratio of the two chemical variants of the mature cross-link, hydroxylysylpyridinoline to lysylpyridinoline, was constant throughout adult life at 3.5:1, whereas in cartilage it was always greater than 10:1. The ratio of hydroxypyridinium cross-links to borohydride-reducible keto-amine cross-links also changed with age. The reducible cross-links in bone collagen decreased steeply in content between birth and 25 years, but persisted in significant amounts throughout adult life. Reducible cross-links had virtually disappeared from cartilage by 10-15 years of age, being replaced by hydroxypyridinium residues, their maturation products. Cancellous and cortical bone collagens showed similar trends with age in their content of mature cross-links, though for each subject the concentration in cancellous bone was always lower than in cortical bone, presumably reflecting the higher turnover rate and hence the more immature state of cancellous bone.
对年龄从1个月到80岁的人类受试者的骨骼和软骨样本中羟吡啶交联氨基酸的胶原蛋白浓度进行了测量。分别对皮质骨和松质骨样本进行了解剖和分析。在骨骼和软骨中，这种成熟交联形式的含量在10至15岁时达到最高值（软骨中的含量是骨骼中的5至10倍），然后在成年期基本保持在相同范围内。在骨骼中，成熟交联的两种化学变体，即羟赖氨酰吡啶啉与赖氨酰吡啶啉的比例在成年期始终保持恒定，为3.5:1，而在软骨中该比例始终大于10:1。羟吡啶交联与硼氢化物可还原的酮胺交联的比例也随年龄变化。骨骼胶原蛋白中可还原交联的含量在出生至25岁之间急剧下降，但在成年期始终大量存在。到10至15岁时，软骨中的可还原交联实际上已消失，被其成熟产物羟吡啶残基所取代。松质骨和皮质骨胶原蛋白中成熟交联的含量随年龄呈现相似趋势，不过对于每个受试者而言，松质骨中的浓度始终低于皮质骨，这可能反映了松质骨的更新率较高，因此其状态更不成熟。
