呼吸作用的牛心亚线粒体颗粒产生的磷酸化电位。
The phosphorylation potential generated by respiring bovine heart submitochondrial particles.
作者信息
Ferguson S J, Sorgato M C
出版信息
Biochem J. 1977 Nov 15;168(2):299-303. doi: 10.1042/bj1680299.
A phosphorylation potential deltaGp, where deltaGp = deltaGo' + RT2.303 log ([ATP]/([ADP][Pi])), of approx. 44.3 kJ.mol-1 (10.6 kcal.mol-1) was generated by submitochondrial particles that were oxidizing either NADH or succinate. Addition of adenylyl imidodiphosphate, which should suppress adenosine triphosphatase activity of any uncoupled particles, did not raise the phosphorylation potential. Raising the Pi concentration slightly increased the magnitude of the value for [ATP]/[ADP], but this did not fully compensate for the increased Pi concentration, so that the phosphorylation potential decreased slightly as the Pi concentration was raised. The phosphorylation potential developed by submitochondrial particles is lower than that generated by phosphorylating membrane vesicles from some bacteria, and is also less than that developed externally by mitochondria, but is strikingly close to the phosphorylation potential that is generated internally by mitochondria.
通过氧化NADH或琥珀酸的亚线粒体颗粒产生了约44.3 kJ·mol⁻¹（10.6 kcal·mol⁻¹）的磷酸化电位ΔGp，其中ΔGp = ΔG°' + RT2.303 log（[ATP]/（[ADP][Pi]））。添加腺苷酰亚胺二磷酸（应抑制任何解偶联颗粒的腺苷三磷酸酶活性）并没有提高磷酸化电位。略微提高Pi浓度会使[ATP]/[ADP]值的幅度略有增加，但这并不能完全补偿增加的Pi浓度，因此随着Pi浓度的升高，磷酸化电位会略有下降。亚线粒体颗粒产生的磷酸化电位低于某些细菌的磷酸化膜囊泡产生的电位，也低于线粒体在外部产生的电位，但与线粒体在内部产生的磷酸化电位惊人地接近。
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