Axe reproduction, santé périnatale et santé de l'enfant, Centre de Recherche en Biologie de la Reproduction (CRBR), Centre de Recherche du Centre Hospitalier de l'Université Laval (CHUQ-CHUL), Québec, Canada.
Placenta. 2010 May;31(5):392-400. doi: 10.1016/j.placenta.2010.02.007. Epub 2010 Mar 11.
A decreased antioxidant activity for superoxide dismutases (SODs) in the placenta was reported in preeclampsia (PE). However, it is unclear if this reduced enzymatic activity can be attributed to a specific SOD isoform. Moreover, the specific spatial SOD expression in the placenta and the impact of the mode of delivery on the latter are still lacking. There are three known SOD isoforms: SOD1 (cytosolic), SOD2 (mitochondrial) and SOD3 (extracellular). Our main objective was to characterize by RT-PCR, western blot and immunolocalization, the expression of SOD1, SOD2, and SOD3 in placentas of normotensive (n = 23) and PE pregnancies (n = 25) according to the presence or absence of labor, the sampling site (peri-insertion, mid-disc and periphery) and the placental layer: amnion-chorion, villi, and maternal side layer (MS). In absence of labor (cesarean), SOD1 expression in the placental villi and MS was lower in PE than in controls (p < 0.049). In presence of labor (vaginal deliveries), SOD1 expression in the amnion-chorion only was higher in PE than controls (p = 0.014). Additionally, SOD2 and SOD3 expression in presence of labor were higher in all three layers in PE than controls, with a strong positive correlation between these two SODs (mRNA; r > 0.65, p < 0.008). The sampling site and gestational age had no effect on SOD expression within the placenta. In this study, we showed that the reported decrease for SOD activity in PE may be attributed to SOD1 in absence of labor. Also, this is the first study characterizing specific SOD isoforms according to the mode of delivery. We demonstrated in PE that labor upregulates SOD1 in fetal membranes as well as SOD2 and SOD3 in the whole placenta.
在子痫前期（PE）中，报道称胎盘中超氧化物歧化酶（SODs）的抗氧化活性降低。然而，目前尚不清楚这种降低的酶活性是否归因于特定的 SOD 同工型。此外，胎盘中特定的 SOD 表达模式以及分娩方式对其的影响仍不清楚。目前已知有三种 SOD 同工型：SOD1（细胞浆）、SOD2（线粒体）和 SOD3（细胞外）。我们的主要目的是通过 RT-PCR、western blot 和免疫定位来描述 SOD1、SOD2 和 SOD3 在正常妊娠（n = 23）和 PE 妊娠（n = 25）胎盘中的表达，根据是否存在分娩、采样部位（附着前、中盘和边缘）和胎盘层：羊膜绒毛膜、绒毛和母体侧层（MS）进行描述。在无分娩（剖宫产）的情况下，PE 胎盘绒毛和 MS 中的 SOD1 表达低于对照组（p < 0.049）。在有分娩（阴道分娩）的情况下，PE 胎盘的羊膜绒毛膜中 SOD1 的表达高于对照组（p = 0.014）。此外，在有分娩的情况下，PE 胎盘的所有三层中的 SOD2 和 SOD3 表达均高于对照组，这两种 SOD 之间存在强烈的正相关（mRNA；r > 0.65，p < 0.008）。采样部位和胎龄对胎盘内 SOD 表达没有影响。在这项研究中，我们表明，PE 中报道的 SOD 活性降低可能归因于无分娩时的 SOD1。此外，这是首次根据分娩方式对特定 SOD 同工型进行特征描述的研究。我们在 PE 中证明，分娩会在上皮中上调 SOD1，以及在整个胎盘上调 SOD2 和 SOD3。
