Centre for Cellular and Molecular Biology, Hyderabad 500007, India.
Dev Biol. 2010 May 15;341(2):389-99. doi: 10.1016/j.ydbio.2010.02.039. Epub 2010 Mar 11.
Animal growth and development is dependent on reiterative use of key signaling pathways such as Hedgehog (Hh) pathway. It is widely believed that Cubitus-interruptus (Ci) mediates all functions of Hh pathway. Here we report that CG32062, the Drosophila homologue of Ataxin-2 Binding Protein 1 (dA2BP1), functions as a cofactor of Ci to specify intervein region between L3 and L4 veins of the adult wing. Specifically, Ci-mediated transactivation of knot/collier (kn) in this region of the developing wing imaginal disc is dependent on dA2BP1 function. Protein interaction studies and chromatin-immunoprecipiation experiments suggest that Ci helps dA2BP1 to bind kn promoter, which in turn may help Ci to activate kn expression. These results suggest a mechanism by which Ci may activate targets such as kn, which do not have classical Ci/Gli-binding sites.
动物的生长和发育依赖于关键信号通路的反复使用，如 Hedgehog（Hh）通路。人们普遍认为 Cubitus-interruptus（Ci）介导 Hh 通路的所有功能。在这里，我们报告说，果蝇同源物 Ataxin-2 Binding Protein 1（dA2BP1）的 CG32062 作为 Ci 的辅助因子，特异性地指定成年翅膀中 L3 和 L4 脉之间的静脉间区域。具体而言，Ci 介导的发育中的翅膀 imaginal 盘这个区域中的结/结（kn）的转录激活依赖于 dA2BP1 的功能。蛋白相互作用研究和染色质免疫沉淀实验表明，Ci 有助于 dA2BP1 结合 kn 启动子，反过来可能有助于 Ci 激活 kn 的表达。这些结果表明 Ci 可能激活靶基因（如 kn）的机制，而这些靶基因没有经典的 Ci/Gli 结合位点。
