Mysore Keshava, Sun Longhua, Hapairai Limb K, Wang Chien-Wei, Roethele Joseph B, Igiede Jessica, Scheel Max P, Scheel Nicholas D, Li Ping, Wei Na, Severson David W, Duman-Scheel Molly
Department of Medical and Molecular Genetics, Indiana University School of Medicine, Raclin-Carmichael Hall, 1234 Notre Dame Ave., South Bend, IN 46617, USA.
Eck Institute for Global Health, The University of Notre Dame, South Bend, IN 46556, USA.
Pathogens. 2021 Sep 28;10(10):1251. doi: 10.3390/pathogens10101251.
Prevention of mosquito-borne infectious diseases will require new classes of environmentally safe insecticides and novel mosquito control technologies. was engineered to express short hairpin RNA (shRNA) corresponding to mosquito genes. The yeast induced target gene silencing, resulting in larval death that was observed in both laboratory and outdoor semi-field trials conducted on . High levels of mortality were also observed during simulated field trials in which adult females consumed yeast delivered through a sugar bait. Mortality correlated with defects in the mosquito brain, in which a role for as a positive regulator of signaling was identified. The larvicidal and adulticidal activities of the yeast were subsequently confirmed in trials conducted on , , and , yet the yeast had no impact on survival of select non-target arthropods. These studies indicate that yeast RNAi pesticides targeting could be further developed as broad-based mosquito larvicides and adulticides for deployment in integrated biorational mosquito control programs. These findings also suggest that the species-specificity of attractive targeted sugar baits, a new paradigm for vector control, could potentially be enhanced through RNAi technology, and specifically through the use of yeast-based interfering RNA pesticides.
预防蚊媒传染病将需要新型的环境安全杀虫剂和新颖的蚊虫控制技术。对[具体名称未给出]进行了工程改造，使其表达与蚊虫基因相对应的短发夹RNA（shRNA）。酵母诱导了靶基因沉默，导致幼虫死亡，这在对[具体名称未给出]进行的实验室和室外半田间试验中均有观察到。在模拟田间试验中，成年雌性蚊子食用通过糖饵传递的酵母时，也观察到了高死亡率。死亡率与蚊虫大脑中的缺陷相关，其中确定了[具体名称未给出]作为[具体名称未给出]信号的正调节因子的作用。随后在对[具体名称未给出]、[具体名称未给出]和[具体名称未给出]进行的试验中证实了酵母的杀幼虫和杀成虫活性，但酵母对选定的非靶节肢动物的存活没有影响。这些研究表明，靶向[具体名称未给出]的酵母RNAi杀虫剂可进一步开发为广泛使用的蚊虫幼虫和成虫杀虫剂，用于综合生物合理蚊虫控制计划。这些发现还表明，有吸引力的靶向糖饵的物种特异性，一种新的病媒控制范式，可能通过RNAi技术，特别是通过使用基于酵母的干扰RNA杀虫剂来潜在地增强。
