Miseta A, Kellermayer M, Ludany A, Cameron I L, Hazlewood C F
Department of Clinical Chemistry, Medical University of Pecs, Hungary.
J Cell Physiol. 1991 Mar;146(3):394-8. doi: 10.1002/jcp.1041460309.
The release of sodium and potassium and the uptake of sucrose molecules was studied in pig lenses incubated in isosmotic sucrose solution in either the presence or absence of 1% Triton X-100 (a non-ionic detergent). This Triton X-100 treatment has been shown to cause severe disruptions of cell membrane integrity. If sodium and potassium were free in the lens fibers as in a dilute aqueous solution, they would be expected to diffuse three to four times faster than sucrose. However, measurements of sodium and potassium release and sucrose uptake in the Triton X-100 treated lenses show a 1:1 equilibration. When pig lenses were incubated in the same solution without detergent, the sucrose uptake was significantly less than the potassium and sodium release. It is postulated that a slow, detergent mediated collapse of protein-water-ion interactions within the lens is the rate-limiting step of the observed equilibration of monovalent cations and sucrose molecules.
在等渗蔗糖溶液中培养的猪晶状体中，研究了在有或没有1% Triton X-100（一种非离子去污剂）存在的情况下钠和钾的释放以及蔗糖分子的摄取。已证明这种Triton X-100处理会导致细胞膜完整性的严重破坏。如果钠和钾在晶状体纤维中像在稀水溶液中一样是自由的，那么它们扩散的速度预计会比蔗糖快三到四倍。然而，对经Triton X-100处理的晶状体中钠和钾释放以及蔗糖摄取的测量显示出1:1的平衡。当猪晶状体在没有去污剂的相同溶液中培养时，蔗糖摄取明显少于钾和钠的释放。据推测，晶状体中蛋白质 - 水 - 离子相互作用的缓慢、去污剂介导的破坏是观察到的单价阳离子和蔗糖分子平衡的限速步骤。
