Johnson M R, Okokon E, Collins W P, Sharma V, Lightman S L
Neuroendocrinology Unit, Charing Cross and Westminster Medical School, Charing Cross Hospital, London, United Kingdom.
J Clin Endocrinol Metab. 1991 May;72(5):1042-7. doi: 10.1210/jcem-72-5-1042.
The effect of hCG and pregnancy on the circulating levels of relaxin was investigated in 48 women undergoing in vitro fertilization and embryo transfer (ET) for the treatment of infertility. Subjects were allocated randomly to receive hCG (Profasi; 2000 IU, im) or placebo on the day of ET (day 0) and on day 3 after ET (day 3). Samples of peripheral blood were taken on days -4, 6, and 10. An additional sample was taken on day 22 from women who became pregnant. The subjects were allocated retrospectively to 1 of 4 groups: no pregnancy, no hCG (NP); pregnancy, no hCG (P); no pregnancy, hCG (NPH); and pregnancy, hCG (PH). Two patients with blighted ova and one with a tubal pregnancy were excluded from the analysis. The concentrations of relaxin were similar and rose significantly in all groups at each time point (P less than 0.05). On day 6 there was no significant difference in the increment between the groups, but by day 10, circulating levels in the P compared to the NP and those in the PH compared to the NPH group were significantly greater (P less than 0.05). By day 22 the difference between the PH and the P groups was significant (P less than 0.05). Relaxin levels correlated with progesterone levels on day 10 in the NP and P groups (r = 0.633; P less than 0.05 and r = 0.697; P less than 0.05, respectively) and with estradiol levels in the P group only on days 6 and 22 (r = 0.659; P less than 0.05 and r = 0.783; P less than 0.05, respectively). These data demonstrate that in women undergoing in vitro fertilization, relaxin levels increase during the luteal phase, and in those women who establish a pregnancy, the values are significantly greater by day 10. The administration of hCG in the early luteal phase, before implantation, to women who subsequently become pregnant significantly increased the level of circulating relaxin on day 22. The positive correlation between relaxin levels and ovarian steroid levels in the groups not receiving exogenous hCG suggests that a common factor may control the release of both.
对48名因不孕症接受体外受精和胚胎移植（ET）的女性，研究了人绒毛膜促性腺激素（hCG）和妊娠对循环中松弛素水平的影响。受试者在胚胎移植日（第0天）和胚胎移植后第3天（第3天）被随机分配接受hCG（普丽康；2000IU，肌肉注射）或安慰剂。在第-4、6和10天采集外周血样本。在第22天从怀孕的女性中额外采集一份样本。受试者被回顾性地分为4组之一：未妊娠，未用hCG（NP）；妊娠，未用hCG（P）；未妊娠，用hCG（NPH）；妊娠，用hCG（PH）。两名胚胎停育患者和一名输卵管妊娠患者被排除在分析之外。所有组中松弛素浓度在每个时间点均相似且显著升高（P<0.05）。在第6天，各组间的升高幅度无显著差异，但到第10天，妊娠组（P）与未妊娠组（NP）相比以及妊娠且用hCG组（PH）与未妊娠且用hCG组（NPH）相比，循环水平显著更高（P<0.05）。到第22天，PH组与P组之间的差异显著（P<0.05）。在未妊娠组（NP）和妊娠组（P）中，第10天时松弛素水平与孕酮水平相关（r=0.633；P<0.05和r=0.697；P<0.05），在妊娠组（P）中仅在第6天和第22天时松弛素水平与雌二醇水平相关（r=0.659；P<0.05和r=0.783；P<0.05）。这些数据表明，在接受体外受精的女性中，黄体期松弛素水平升高，在成功妊娠的女性中，到第10天时其值显著更高。在植入前的黄体早期对随后怀孕的女性给予hCG，显著增加了第22天时循环中松弛素的水平。在未接受外源性hCG的组中，松弛素水平与卵巢甾体激素水平之间的正相关表明可能有一个共同因素控制两者的释放。
