Wechsler S J, McHolland L E, Wilson W C
U.S. Department of Agriculture, Arthropod-Borne Animal Diseases Research Laboratory, Laramie, Wyoming 82071-3965.
J Invertebr Pathol. 1991 Mar;57(2):200-5. doi: 10.1016/0022-2011(91)90117-9.
A virus was detected in cells (designated CuVa) cultured from one laboratory colony of the biting midge, Culicoides variipennis. By electron microscopy (30 nm), nonenveloped, icosahedral virions arranged separately and in crystalline matrix arrays were seen in the cytoplasm but not in the nucleus of CuVa cells. Separation by 10% polyacrylamide gel electrophoresis revealed multiple bands of viral-induced double-stranded RNA. Inoculation of this virus onto different cell lines and intracranially into suckling mice revealed no detectable pathology. Immunoperoxidase staining using polyclonal antibody determined that the virus is infectious to toad cells, bovine endothelial cells, bovine kidney cells, mosquito cells, and cells (designated KC) initiated from another laboratory colony of C. variipennis. KC cells infected with this virus were coinfected with bluetongue virus with no decrease in bluetongue virus titer.
在从库蠓（Culicoides variipennis）的一个实验室群体培养的细胞（命名为CuVa）中检测到一种病毒。通过电子显微镜（30纳米）观察，在CuVa细胞的细胞质中可见无包膜的二十面体病毒粒子单独存在并呈晶体基质排列，而在细胞核中未见。10%聚丙烯酰胺凝胶电泳分离显示出病毒诱导的双链RNA的多条条带。将这种病毒接种到不同细胞系并颅内接种到乳鼠中，未发现可检测到的病理学变化。使用多克隆抗体的免疫过氧化物酶染色确定该病毒可感染蟾蜍细胞、牛内皮细胞、牛肾细胞、蚊子细胞以及从库蠓的另一个实验室群体起始的细胞（命名为KC）。感染这种病毒的KC细胞与蓝舌病毒共同感染，蓝舌病毒滴度未降低。
