Wechsler S J, McHolland L E, Tabachnick W J
J Invertebr Pathol. 1989 Nov;54(3):385-93. doi: 10.1016/0022-2011(89)90123-7.
Cell lines have been developed from 2-day-old embryos of the biting midge, Culicoides variipennis (Diptera: Ceratopogonidae). In North America C. variipennis is the primary insect vector of bluetongue virus (BTV), an orbivirus that causes disease of ruminants. The C. variipennis (CuVa) cells, grown in Schneider's Drosophila medium, consist primarily of a fibroblast-like cell type. CuVa cells are very hardy. They can grow over a wide range of temperature and pH and adapt to growth in minimal essential medium. BTV replicates to high titer (7.5-8.0 log10 50% tissue culture infectious doses/ml) in CuVa cells over a wide range of temperatures (19 degrees to 37 degrees C) without inducing any significant cytopathic effects. The highest BTV titers were obtained in CuVa cells grown at 25 degrees and 32 degrees C. Cells from C. variipennis can be useful for many diverse investigations.
细胞系已从2日龄的刺吸蠓（Culicoides variipennis，双翅目：蠓科）胚胎中建立。在北美，C. variipennis是蓝舌病毒（BTV）的主要昆虫传播媒介，BTV是一种引起反刍动物疾病的环状病毒。在施奈德果蝇培养基中生长的C. variipennis（CuVa）细胞主要由成纤维细胞样细胞类型组成。CuVa细胞非常耐寒。它们能在很宽的温度和pH范围内生长，并能适应在最低限度基本培养基中的生长。BTV在很宽的温度范围（19℃至37℃）内于CuVa细胞中复制至高效价（7.5 - 8.0 log10 50%组织培养感染剂量/毫升），且不产生任何明显的细胞病变效应。在25℃和32℃下生长的CuVa细胞中获得了最高的BTV效价。C. variipennis的细胞可用于许多不同的研究。
