Leonard E J, Yoshimura T, Tanaka S, Raffeld M
Immunopathology Section, National Cancer Institute, Frederick, Maryland 21702.
J Invest Dermatol. 1991 May;96(5):690-4. doi: 10.1111/1523-1747.ep12470612.
Neutrophil attractant/activation protein-1 (NAP-1) is a recently described cytokine that attracts neutrophils, but not monocytes or eosinophils. This leukocyte specificity is not absolute, in that NAP-1 attracts basophils and small numbers of lymphocytes. Our purpose was to determine in vivo effects of NAP-1, and to compare them to the reported action of the complement attractant, C5a. Intradermal injection into normal human subjects of 40 microliters of NAP-1, over a concentration range of 4 x 10(-8) M to 10(-6) M, caused no symptoms or signs such as wheal-and-flare, itching, induration, or tenderness. However, biopsies of injection sites showed perivascular neutrophil infiltration as early as 30 min, which increased at 1 and 3 h. The mean number of neutrophils per mm2 of dermis for 15 biopsies taken 3 h after intradermal injection of 2 x 10(-7) M or 10(-6) M NAP-1 was 164 +/- 41; the response to saline or a NAP-1 inactive fragment was 5 or less. Intradermal NAP-1 did not cause basophil or lymphocyte infiltration. Consistent with the absence of a wheal-and-flare, acid toluidine blue-stained sections showed no evidence of mast cell degranulation, in contrast to previously reported results with C5a. Thus, the predominant response by human subjects to intradermal NAP-1 was neutrophil accumulation in proximity to dermal blood vessels.
中性粒细胞趋化因子/激活蛋白-1（NAP-1）是最近发现的一种细胞因子，它可吸引中性粒细胞，但不能吸引单核细胞或嗜酸性粒细胞。这种白细胞特异性并非绝对，因为NAP-1可吸引嗜碱性粒细胞和少量淋巴细胞。我们的目的是确定NAP-1在体内的作用，并将其与补体趋化因子C5a的已知作用进行比较。在4×10⁻⁸M至10⁻⁶M的浓度范围内，向正常人体受试者皮内注射40微升NAP-1，未引起诸如风团和潮红、瘙痒、硬结或压痛等症状或体征。然而，注射部位的活检显示，早在30分钟时就有血管周围中性粒细胞浸润，在1小时和3小时时增加。皮内注射2×10⁻⁷M或10⁻⁶M NAP-1 3小时后，15份活检标本中每平方毫米真皮的中性粒细胞平均数量为164±41；对生理盐水或NAP-1无活性片段的反应为5或更少。皮内注射NAP-1未引起嗜碱性粒细胞或淋巴细胞浸润。与没有风团和潮红一致，酸性甲苯胺蓝染色切片未显示肥大细胞脱颗粒的证据，这与先前报道的C5a的结果形成对比。因此，人体受试者对皮内注射NAP-1的主要反应是真皮血管附近的中性粒细胞聚集。
