Wellman Center for Photomedicine, Department of Dermatology, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA.
Nat Methods. 2010 Apr;7(4):303-5. doi: 10.1038/nmeth.1440. Epub 2010 Mar 14.
In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts in mice and may be extensible to imaging organ parenchyma such as cerebral cortex. We monitored cell infiltration, vascular changes and tumor progression during inflammation and tumorigenesis in colon over several months.
小动物体内成像为研究正常和疾病生物学提供了多种可能性，但实现单细胞分辨率的器官可视化具有挑战性。我们描述了旋转侧视共聚焦内窥技术，该技术可实现对小鼠胃肠道和呼吸道的细胞成像，并且可能可扩展到对脑皮质等器官实质的成像。我们在几个月的时间内监测了炎症和肿瘤发生过程中结肠中的细胞浸润、血管变化和肿瘤进展。
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