College of Animal Sciences, Zhejiang University, 310029, Hangzhou, China.
Biotechnol Lett. 2010 Jul;32(7):963-7. doi: 10.1007/s10529-010-0241-9. Epub 2010 Mar 13.
To elucidate the effects of C-terminal domains of LicMB (mature lichenase from Clostridium thermocellum) and terminal residues of LicMB-CD (catalytic domain of LicMB) on the properties of lichenase, a series of truncated genes were constructed and expressed in E. coli. The Thr-Pro box had a positive effect while the dockerin domain had a negative impact on the properties of LicMB. The N-terminal 10-25th and C-terminal 1-9th residues of LicMB-CD were necessary to retain high thermostability while the N-terminal 1-7th and C-terminal 1-3rd residues were not necessary to maintain enzymatic activity.
为了阐明来自热纤梭菌的 C 端结构域的 LicMB（成熟的几丁质酶）和 LicMB-CD（LicMB 的催化结构域）的末端残基对几丁质酶性质的影响，构建了一系列截短基因，并在大肠杆菌中进行表达。Thr-Pro 盒具有积极影响，而 dockerin 结构域对 LicMB 的性质具有负面影响。LicMB-CD 的 N 端 10-25 位和 C 端 1-9 位残基是保留高耐热性所必需的，而 N 端 1-7 位和 C 端 1-3 位残基对于保持酶活性不是必需的。
