Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, 24106 North Bunn Road, Prosser, WA 99350, USA.
Arch Virol. 2010 May;155(5):643-56. doi: 10.1007/s00705-010-0630-3. Epub 2010 Mar 13.
Two new 'legumoviruses' (genus Begomovirus; family Geminiviridae) naturally infecting soybean (Glycine max L. Merr.) in Nigeria were molecularly characterized. Based on characteristic symptoms in soybean, the two viruses are provisionally designated as Soybean mild mottle virus (SbMMV) and Soybean chlorotic blotch virus (SbCBV). SbCBV has a bipartite genome, whereas SbMMV has only a DNA A component. The DNA A component of SbMMV is 2,768 nucleotides (nt) long and the DNA A and DNA B components of SbCBV are 2,708 and 2,647 nt long, respectively. In pairwise comparisons, the DNA A component of SbMMV and SbCBV showed 62% nt sequence identity, indicating that these two viruses are distinct. Whereas the DNA A of SbMMV contains two virion- and four complementary-sense open reading frames, that of SbCBV lacks the virus-sense AV2, a signature gene present in 'Old World' begomoviruses. A pairwise comparison with the corresponding nucleotide sequence of other begomoviruses in the databases indicated that SbCBV had a maximum of 74% identity with cowpea golden mosaic virus and SbMMV had a maximum of 65% identity with mungbean yellow mosaic India virus and kudzu mosaic virus. Phylogenetic analysis of the DNA A component of SbCBV and SbMMV together with those of other begomoviruses available in the databases showed clustering of the two viruses within the 'legumovirus' clade of the begomovirus phylogenetic tree. In addition, the DNA A and B components of SbCBV from Centrosema pubescens Benth were found to be identical to those from soybean, indicating that leguminous wild species are a potential alternative host for the virus. Since soybean is an introduced crop, the identification of two distinct begomoviruses naturally infecting soybean in Nigeria suggests the occurrence of 'legumoviruses' in plant species indigenous to Africa and underscores their potential threat to sustainable cultivation of soybean on the African continent.
两种新的“菜豆金色花叶病毒”（双生病毒科，菜豆花叶病毒属）在尼日利亚自然感染大豆（Glycine max L. Merr.），其分子特征被鉴定。基于大豆的特征症状，这两种病毒暂被命名为大豆轻度花叶病毒（SbMMV）和大豆黄斑驳病毒（SbCBV）。SbCBV 具有二分体基因组，而 SbMMV 只有一个 DNA A 组份。SbMMV 的 DNA A 组份长 2,768 个核苷酸（nt），而 SbCBV 的 DNA A 和 DNA B 组份分别长 2,708 和 2,647 nt。在两两比较中，SbMMV 和 SbCBV 的 DNA A 组份显示 62%的 nt 序列同一性，表明这两种病毒是不同的。虽然 SbMMV 的 DNA A 包含两个病毒和四个互补 sense 的开放阅读框，但 SbCBV 缺乏病毒 sense 的 AV2，这是“旧世界”菜豆花叶病毒属病毒中的一个特征基因。与数据库中其他菜豆花叶病毒属病毒的相应核苷酸序列进行两两比较表明，SbCBV 与豇豆花叶病毒的同源性最高可达 74%，而 SbMMV 与绿豆黄花叶印度病毒和木薯花叶病毒的同源性最高可达 65%。对 SbCBV 和 SbMMV 的 DNA A 组份以及数据库中其他菜豆花叶病毒属病毒的 DNA A 组份进行系统进化分析表明，这两种病毒聚类在菜豆花叶病毒属病毒的系统进化树的“菜豆病毒”分支内。此外，从 Centrosema pubescens Benth 分离的 SbCBV 的 DNA A 和 B 组份与大豆中的相同，表明豆科野生种是该病毒的潜在替代寄主。由于大豆是一种引进作物，在尼日利亚自然感染大豆的两种独特的菜豆花叶病毒的鉴定表明，“菜豆病毒”在非洲本土植物物种中存在，并强调了它们对非洲大陆可持续种植大豆的潜在威胁。
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