Wang Y, Liu H, Zhang Y, Ma D
Laboratory of Medical Immunology, Beijing Medical University, 100083, Beijing, China.
Sci China C Life Sci. 1999 Jun;42(3):323-9. doi: 10.1007/BF03183610.
By means of cDNA-RDA method, some cDNA fragments were found to have high levels of expression during deprivation of GM-CSF (granulocyte macrophage-colony stimulating factor) in a human myeloid cell line, TF-1 cells. One of these fragments was identified as a novel gene. To get the full length of cDNA, rapid amplification of cDNA ends (RACE) and expressed sequence tags (EST) overlapping fragments assembling strategies were used. The novel gene was named TRAF15 (TF-1 cell apoptosis related gene-15), which consists of 1 218 nucleotides and encodes 212 amino acids. The putative protein product of TFAR15 is partially homologous toC. elegans protein C14A4.11. TFAR15 mRNA is expressed in fetal liver, kidney, spleen and lung, and also in some human myeloid cell lines. Both of the TFAR15 mRNA and protein were highly expressed in TF-1 cells after GM-CSF withdrawal.In vitro analysis showed that the recombinant TFAR15 protein could inhibit the natural cell death of 293 cells, an embryonic kidney cell line.
通过cDNA-RDA方法，发现在人髓样细胞系TF-1细胞中GM-CSF（粒细胞巨噬细胞集落刺激因子）缺失期间，一些cDNA片段具有高水平表达。其中一个片段被鉴定为一个新基因。为了获得cDNA全长，采用了cDNA末端快速扩增（RACE）和表达序列标签（EST）重叠片段拼接策略。该新基因被命名为TRAF15（TF-1细胞凋亡相关基因-15），它由1218个核苷酸组成，编码212个氨基酸。TFAR15的推定蛋白产物与秀丽隐杆线虫蛋白C14A4.11部分同源。TFAR15 mRNA在胎儿肝脏、肾脏、脾脏和肺中表达，也在一些人髓样细胞系中表达。GM-CSF撤除后，TFAR15 mRNA和蛋白在TF-1细胞中均高表达。体外分析表明，重组TFAR15蛋白可抑制胚胎肾细胞系293细胞的自然细胞死亡。
