Center of Herbal Resources Research, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
Immunopharmacol Immunotoxicol. 2010 Dec;32(4):663-70. doi: 10.3109/08923971003671108. Epub 2010 Mar 15.
Glehnia littoralis (Umbelliferae) is a traditional medicine used in Korea, China, and Japan to treat the immune-related diseases. However, its anti-inflammatory activities and mechanisms remain to be defined. We investigated the effects of 70% ethanolic extract from G. littoralis (GLE) on skin inflammation in mice. Production of proinflammatory cytokines (IL-1β and TNF-α), activation of myeloperoxidase (MPO), and histological indicators were examined in acute and chronic skin inflammation using 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced mouse ear edema. We also performed acetic acid-induced vascular permeability tests. GLE treatment at 200 mg/kg inhibited topical edema in the mouse ear, leading to substantial reductions in skin thickness and tissue weight, inflammatory cytokine production, neutrophil-mediated MPO activity, and several histopathological indicators. Furthermore, GLE effectively reduced inflammatory damage induced by chronic TPA exposure and significantly inhibited the vascular permeability induced by acetic acid in mice. These results suggest that G. littoralis is an effective anti-inflammatory agent in murine phorbol ester-induced dermatitis and may have therapeutic potential in a variety of immune-related cutaneous diseases.
珊瑚菜（伞形科）是一种传统药物，在韩国、中国和日本被用于治疗与免疫相关的疾病。然而，其抗炎活性和机制仍有待确定。我们研究了珊瑚菜 70%乙醇提取物（GLE）对小鼠皮肤炎症的影响。使用 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的小鼠耳水肿，在急性和慢性皮肤炎症中检测了促炎细胞因子（IL-1β和 TNF-α）的产生、髓过氧化物酶（MPO）的激活以及组织学指标。我们还进行了醋酸诱导的血管通透性试验。GLE 以 200mg/kg 剂量处理可抑制小鼠耳部的局部水肿，导致皮肤厚度和组织重量、炎症细胞因子产生、中性粒细胞介导的 MPO 活性以及几种组织病理学指标显著降低。此外，GLE 可有效减轻慢性 TPA 暴露引起的炎症损伤，并显著抑制小鼠醋酸诱导的血管通透性。这些结果表明，珊瑚菜是一种有效的鼠佛波酯诱导性皮炎抗炎剂，可能对多种与免疫相关的皮肤疾病具有治疗潜力。
