Nutrition and Health Department, Nestlé Research Center, Lausanne, Switzerland.
Rejuvenation Res. 2010 Feb;13(1):47-53. doi: 10.1089/rej.2009.0895.
Aging is associated with alterations of immune responses. Wolfberry, a popular Chinese functional ingredient, is prized for its anti-aging properties; however, little is known about the immunological effect of wolfberry intake. The purpose of this study was to examine the effect of dietary intake of a milk-based formulation of wolfberry, named Lacto-Wolfberry, on in vivo and ex vivo parameters of adaptive immunity in young-adult and aged mice. Over 44 days, young-adult (2 months) and aged (21 months) C57BL/6J mice were fed ad libitum with a controlled diet and received drinking water supplemented or not with 0.5% (wt/vol) Lacto-Wolfberry. All mice were immunized on day 15 and challenged on day 22 with a T cell- dependent antigen, keyhole limpet hemocyanin (KLH). Lacto-Wolfberry supplementation significantly increased in vivo systemic immune markers that are known to decline with aging. Indeed, both antigen-(KLH) specific humoral response and cell-mediated immune responses in young-adult and aged mice were enhanced when compared to their respective controls. No significant effect of Lacto-Wolfberry supplementation was observed on ex vivo spleen cells proliferative response to mitogens and on splenocyte T cell subsets. In conclusion, dietary intake of Lacto-Wolfberry may favorably modulate the poor responsiveness to antigenic challenge observed with aging.
衰老是与免疫反应的改变相关的。枸杞，一种流行的中国功能性成分，因其抗衰老特性而备受推崇；然而，关于枸杞摄入的免疫学效应知之甚少。本研究的目的是研究食用一种名为 Lacto-Wolfberry 的基于牛奶的枸杞配方对年轻成年和老年小鼠体内和体外适应性免疫参数的影响。在 44 天的时间里，年轻成年（2 个月）和老年（21 个月）C57BL/6J 小鼠自由喂食控制饮食，并接受补充或不补充 0.5%（wt/vol）Lacto-Wolfberry 的饮用水。所有小鼠在第 15 天接受免疫接种，并在第 22 天用 T 细胞依赖性抗原，血蓝蛋白（KLH）进行攻毒。Lacto-Wolfberry 补充剂显著增加了体内已知随衰老而下降的系统性免疫标志物。事实上，与各自的对照相比，年轻成年和老年小鼠的抗原（KLH）特异性体液反应和细胞介导的免疫反应都得到了增强。Lacto-Wolfberry 补充剂对丝裂原刺激的体外脾细胞增殖反应和脾细胞 T 细胞亚群没有显著影响。总之，食用 Lacto-Wolfberry 可能有利于调节与衰老相关的抗原挑战反应不佳的情况。
