Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, 266071, Qingdao, China.
Fish Physiol Biochem. 2022 Aug;48(4):1057-1073. doi: 10.1007/s10695-022-01102-3. Epub 2022 Jul 14.
Krill oil (KO) extracted from Antarctic krill (Euphausia superba) mainly comprises phospholipids and triglycerides. KO has been shown to prolong the median lifespan of the nematode Caenorhabditis elegans, but to shorten the lifespan of long-lived F1 mice; therefore, it remains controversial over the life-extending property of KO. In this study, we clearly demonstrated that dietary intake of KO extended both the mean and maximum lifespans of aged male Nothobranchius guentheri (p < 0.05), reduced the accumulation of lipofuscin (LF) (p < 0.05) in the gills and senescence-associated β-galactosidase (SA-β-Gal) (p < 0.05) in the caudal fins, and lowered the levels of protein oxidation (p < 0.05), lipid peroxidation (p < 0.01), and reactive oxygen species (ROS) (p < 0.01) in the muscles and livers, indicating that KO possesses rejuvenation and anti-aging activity. We also showed that KO enhanced the activities of antioxidant enzymes catalase (CAT) (p < 0.05), superoxide dismutase (SOD) (p < 0.05), and glutathione peroxidase (GPX) (p < 0.05) in aged male N. guentheri. In addition, KO administration effectively reversed histological lesions including inflammatory cell infiltration and structural collapse in the muscles and livers of aged N. guentheri and suppressed the nuclear factor kappa-B (NF-κB) signaling pathway (p < 0.05), a master regulator of inflammation. Altogether, our study indicates that KO has anti-aging and rejuvenation property. It also suggests that KO exerts its anti-aging and rejuvenation effects via enhancement of the antioxidant system and suppression of the NF-κB signaling pathway.
南极磷虾油（KO）从南极磷虾（Euphausia superba）中提取，主要包含磷脂和甘油三酯。KO 已被证明能延长秀丽隐杆线虫（Caenorhabditis elegans）的中位寿命，但能缩短长寿 F1 小鼠的寿命；因此，KO 的延长寿命作用仍存在争议。在这项研究中，我们明确表明，KO 的饮食摄入延长了年老雄性 Nothobranchius guentheri 的平均和最大寿命（p<0.05），减少了鳃中脂褐素（LF）（p<0.05）和尾部鳍中衰老相关的β-半乳糖苷酶（SA-β-Gal）（p<0.05）的积累，并降低了肌肉和肝脏中的蛋白质氧化（p<0.05）、脂质过氧化（p<0.01）和活性氧（ROS）（p<0.01）水平，表明 KO 具有抗衰老和抗老化作用。我们还表明，KO 增强了年老雄性 N. guentheri 中抗氧化酶过氧化氢酶（CAT）（p<0.05）、超氧化物歧化酶（SOD）（p<0.05）和谷胱甘肽过氧化物酶（GPX）（p<0.05）的活性。此外，KO 给药有效逆转了年老 N. guentheri 肌肉和肝脏中的组织病变，包括炎症细胞浸润和结构崩溃，并抑制了核因子 kappa-B（NF-κB）信号通路（p<0.05），该通路是炎症的主要调控因子。总之，我们的研究表明 KO 具有抗衰老和返老还童的特性。它还表明，KO 通过增强抗氧化系统和抑制 NF-κB 信号通路发挥其抗衰老和返老还童作用。
